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首页> 外文期刊>Journal of bacteriology >Roles of Coenzyme F420-Reducing Hydrogenases and Hydrogen- and F420-Dependent Methylenetetrahydromethanopterin Dehydrogenases in Reduction of F420 and Production of Hydrogen during Methanogenesis
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Roles of Coenzyme F420-Reducing Hydrogenases and Hydrogen- and F420-Dependent Methylenetetrahydromethanopterin Dehydrogenases in Reduction of F420 and Production of Hydrogen during Methanogenesis

机译：甲烷分解过程中辅酶F420还原氢酶以及依赖于氢和F420的亚甲基四氢甲蝶呤脱氢酶在还原F420和产氢中的作用

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Reduced coenzyme F420 (F420H2) is an essential intermediate in methanogenesis from CO2. During methanogenesis from H2 and CO2, F420H2 is provided by the action of F420-reducing hydrogenases. However, an alternative pathway has been proposed, where H2-dependent methylenetetrahydromethanopterin dehydrogenase (Hmd) and F420H2-dependent methylenetetrahydromethanopterin dehydrogenase (Mtd) together reduce F420 with H2. Here we report the construction of mutants of Methanococcus maripaludis that are defective in each putative pathway. Their analysis demonstrates that either pathway supports growth on H2 and CO2. Furthermore, we show that during growth on formate instead of H2, where F420H2 is a direct product of formate oxidation, H2 production occurs. H2 presumably arises from the oxidation of F420H2, and the analysis of the mutants during growth on formate suggests that this too can occur by either pathway. We designate the alternative pathway for the interconversion of H2 and F420H2 the Hmd-Mtd cycle.
机译：还原型辅酶F 420 （F 420 H 2 ）是CO 2 甲烷生成中必不可少的中间体。在H 2 和CO 2 的甲烷生成过程中，F 420 H 2 通过F 420 还原性氢酶。但是，已经提出了另一种途径，其中依赖H 2 的亚甲基四氢甲蝶呤脱氢酶（Hmd）和依赖F 420 H 2 的亚甲基四氢甲蝶呤脱氢酶（ Mtd）与H 2 一起还原F 420 。在这里，我们报道了在每个推定途径中有缺陷的马氏甲烷球菌突变体的构建。他们的分析表明，这两种途径均支持H 2 和CO 2 的生长。此外，我们表明在甲酸盐而不是H 2 的生长过程中，其中F 420 H 2 是甲酸盐氧化的直接产物，H < sub> 2 产生。 H 2 大概是由F 420 H 2 的氧化产生的，对甲酸盐生长期间突变体的分析表明，这也可能发生通过任一途径。我们指定了Hmd-Mtd循环中H 2 和F 420 H 2 相互转换的替代途径。

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