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首页> 外文期刊>Journal of bacteriology >Competence for Genetic Transformation in Streptococcus pneumoniae: Termination of Activity of the Alternative Sigma Factor ComX Is Independent of Proteolysis of ComX and ComW
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Competence for Genetic Transformation in Streptococcus pneumoniae: Termination of Activity of the Alternative Sigma Factor ComX Is Independent of Proteolysis of ComX and ComW

机译：肺炎链球菌的遗传转化能力：替代Sigma因子ComX活性的终止独立于ComX和ComW的蛋白水解

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摘要

Competence for genetic transformation in Streptococcus pneumoniae is a transient physiological state whose development is coordinated by a peptide pheromone (CSP) and its receptor, which activates transcription of two downstream genes, comX and comW, and 15 other “early” genes. ComX, a transient alternative sigma factor, drives transcription of “late” genes, many of which are essential for transformation. In vivo, ComW both stabilizes ComX against proteolysis by the ClpE-ClpP protease and stimulates its activity. Interestingly, stabilization of ComX by deletion of the gene encoding the ClpP protease did not extend the period of competence. We considered the hypothesis that the rapid decay of competence arises from a rapid loss of ComW and thus of its ComX stimulating activity, so that ComX might persist but lose its transcriptional activity. Western analysis revealed that ComW is indeed a transient protein, which is also stabilized by deletion of the gene encoding the ClpP protease. However, stabilizing both ComX and ComW did not prolong either ComX activity or the period of transformation, indicating that termination of the transcriptional activity of ComX is not dependent on proteolysis of ComW.
机译：肺炎链球菌的遗传转化能力是一种短暂的生理状态，其发展由肽信息素（CSP）及其受体协调，从而激活两个下游基因 comX 的转录和 comW 以及其他15个“早期”基因。 ComX是一种短暂的替代sigma因子，可驱动“晚期”基因的转录，其中许多基因对于转化至关重要。在体内，ComW都能稳定ComX抵抗ClpE-ClpP蛋白酶引起的蛋白水解并刺激其活性。有趣的是，通过缺失编码ClpP蛋白酶的基因来稳定ComX不会延长竞争期限。我们考虑了一个假设，即能力的快速衰减是由于ComW的快速丧失而引起的，因而是其ComX刺激活性的迅速丧失，因此ComX可能会持续存在但会丧失其转录活性。 Western分析表明，ComW确实是一种瞬时蛋白，也可以通过缺失编码ClpP蛋白酶的基因来稳定。但是，稳定ComX和ComW都不会延长ComX活性或转化时间，这表明ComX转录活性的终止不依赖于ComW的蛋白水解。

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