...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >The Burkholderia cenocepacia LysR-Type Transcriptional Regulator ShvR Influences Expression of Quorum-Sensing, Protease, Type II Secretion, and afc Genes
【24h】

The Burkholderia cenocepacia LysR-Type Transcriptional Regulator ShvR Influences Expression of Quorum-Sensing, Protease, Type II Secretion, and afc Genes

机译：Burkholderia cenocepacia LysR型转录调节因子ShvR影响群体感应，蛋白酶，II型分泌和afc基因的表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Burkholderia cenocepacia is a significant opportunistic pathogen in individuals with cystic fibrosis. ShvR, a LysR-type transcriptional regulator, has previously been shown to influence colony morphology, biofilm formation, virulence in plant and animal infection models, and some quorum-sensing-dependent phenotypes. In the present study, it was shown that ShvR negatively regulates its own expression, as is typical for LysR-type regulators. The production of quorum-sensing signal molecules was detected earlier in growth in the shvR mutant than in the wild type, and ShvR repressed expression of the quorum-sensing regulatory genes cepIR and cciIR. Microarray analysis and transcriptional fusions revealed that ShvR regulated over 1,000 genes, including the zinc metalloproteases zmpA and zmpB. The shvR mutant displayed increased gene expression of the type II secretion system and significantly increased protease and lipase activities. Both ShvR and CepR influence expression of a 24-kb genomic region adjacent to shvR that includes the afcA and afcC operons, required for the production of an antifungal agent; however, the reduction in expression was substantially greater in the shvR mutant than in the cepR mutant. Only the shvR mutation resulted in reduced antifungal activity against Rhizoctonia solani. ShvR, but not CepR, was shown to directly regulate expression of the afcA and afcC promoters. In summary, ShvR was determined to have a significant influence on the expression of quorum-sensing, protease, lipase, type II secretion, and afc genes.
机译：伯克霍尔德氏菌是囊性纤维化患者的重要机会病原体。 ShvR，一种LysR型转录调节因子，先前已证明会影响菌落形态，生物膜形成，动植物感染模型中的毒力以及一些群体感应依赖性表型。在本研究中，已显示ShvR负调节其自身表达，如LysR型调节子的典型特征。与野生型相比，在 shvR 突变体的生长中检测到群体感应信号分子的产生更早，并且ShvR抑制了群体感应调节基因 cepIR 和 cciIR 。微阵列分析和转录融合表明，ShvR调节了1,000多个基因，包括锌金属蛋白酶 zmpA 和 zmpB 。 shvR 突变体显示II型分泌系统的基因表达增加，并且蛋白酶和脂肪酶活性显着增加。 ShvR和CepR都影响与 shvR 相邻的24 kb基因组区域的表达，该区域包括 afcA 和 afcC 操纵子，是产生抗真菌剂;但是， shvR 突变体的表达降低要明显大于 cepR 突变体。仅 shvR 突变导致对 Rhizoctonia solani的抗真菌活性降低。 ShvR而非CepR可以直接调节 afcA 和 afcC 启动子。总之，ShvR被确定对群体感应，蛋白酶，脂肪酶，II型分泌和 afc 基因的表达有重要影响。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号