CrgA Is an Inducible LysR-Type Regulator of Neisseria meningitidis, Acting both as a Repressor and as an Activator of Gene Transcription

Raffaele Ieva; Cristina Alaimo; Isabel Delany; Gunther Spohn; Rino Rappuoli; Vincenzo Scarlato

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CrgA Is an Inducible LysR-Type Regulator of Neisseria meningitidis, Acting both as a Repressor and as an Activator of Gene Transcription

机译：CrgA是脑膜炎奈瑟氏球菌的诱导型LysR型调节子，既可以作为基因转录的阻遏物，又可以作为基因转录激活物。

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The crgA gene of Neisseria meningitidis, which codes for a LysR-type regulator, is divergently oriented with respect to the mdaB gene, which codes for a hypothetical NADPH-quinone oxidoreductase. Transcriptional studies of the intergenic region between crgA and mdaB showed that two overlapping and divergent promoters, P_crgA and P_mdaB, control transcription of these genes. Deletion of the crgA gene led to a strong increase in transcription from the P_crgA promoter and a concomitant strong decrease in transcription from the P_mdaB promoter, indicating that CrgA acts both as an autorepressor of transcription at its own promoter and as an activator of transcription at the mdaB promoter. Addition of α-methylene-γ-butyrolactone (MBL), an inducer of NADPH-quinone oxidoreductase, to wild-type N. meningitidis cells specifically resulted in further activation of transcription of the P_mdaB promoter and more repression of transcription of the P_crgA promoter. No such regulation was observed when MBL was added to crgA-deficient cells, indicating that the transcriptional response to MBL is CrgA mediated. Under the same experimental conditions, no regulation of transcription by either CrgA or MBL was detected at the pilus and capsule genes. The role of CrgA in the regulation of gene expression during the infectious cycle of N. meningitidis is discussed.

机译：脑膜炎奈瑟氏球菌的 crgA 基因编码LysR型调节子，相对于 mdaB 基因编码。假设的NADPH-醌氧化还原酶。对 crgA 和 mdaB 之间基因间区域的转录研究表明，两个重叠且不同的启动子P _{crgA 和P _{mdaB 控制这些基因的转录。 crgA 基因的缺失导致P _{crgA 启动子的转录大量增加，同时P _{sub的转录也随之大量减少> mdaB 启动子，表明CrgA在其自身的启动子处既充当转录的自动阻遏物，又在 mdaB 启动子处充当转录的激活物。将NADPH-醌氧化还原酶的诱导剂α-亚甲基-γ-丁内酯（MBL）添加到野生型 N中。脑膜炎细胞特异性地导致P _{mdaB 启动子的转录进一步激活和P _{crgA 转录的更多抑制> 启动子。当将MBL添加到 crgA 缺陷细胞中时，未观察到这种调节，表明对MBL的转录反应是CrgA介导的。在相同的实验条件下，在菌毛和荚膜基因上均未检测到CrgA或MBL的转录调控。 CrgA在 N感染周期中调控基因表达的作用。讨论了脑膜炎。}}}}}} 展开▼

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《Journal of bacteriology》 |2005年第10期|共10页

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Raffaele Ieva; Cristina Alaimo; Isabel Delany; Gunther Spohn; Rino Rappuoli; Vincenzo Scarlato;
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中图分类微生物学;

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专利

1. The phosphocarrier protein HPr of Neisseria meningitidis interacts with the transcription regulator CrgA and its deletion affects capsule production, cell adhesion, and virulence [J] . Derkaoui Meriem, Antunes Ana, Poncet Sandrine, Molecular Microbiology . 2016,第5期

机译：脑膜炎奈瑟氏球菌的磷酸载体蛋白HPr与转录调节剂CrgA相互作用，其缺失会影响胶囊的产生，细胞粘附和毒力

2. The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators [J] . Sainsbury S, Lane LA, Ren J, Nucleic Acids Research . 2009,第14期

机译：脑膜炎奈瑟氏球菌的CrgA结构揭示了LysR转录调节子的新八聚体组装状态

3. The structure of CrgA from Neisseria meningitidis reveals a new octameric assembly state for LysR transcriptional regulators [J] . Carol V. Robinson, David I. Stuart, Jingshan Ren, Nucleic acids research . 2009,第14期

机译：脑膜炎奈瑟氏球菌的CrgA结构揭示了LysR转录调节子的新八聚体组装状态

4. Investigation of iron-limitation inducible proteins of Neisseria meningitidis using the isobaric labelling approach tandem mass tags (TMT) [C] . Thorsten Prinz, Karsten Kuhn, Harald Legner, ASMS Conference on Mass Spectrometry and Allied Topics . 2008

机译：使用等离标记方法串联质量标签（TMT）对丝晶型脑膜炎的铁限制诱导蛋白的调查（TMT）

5. A Tale of Two Regulators: Expression of the Virulence-Associated Type IV Pili and Lipooligosaccharide sialyltransferase is Controlled by CrgA and Rsp in Neisseria gonorrhoeae. [D] . Matthias, Kathryn A. 2013

机译：两种调节物的故事：淋病奈瑟氏球菌中与CrgA和Rsp有关的毒力相关的IV型菌毛和脂寡糖唾液酸转移酶的表达。

6. CrgA Is an Inducible LysR-Type Regulator of Neisseria meningitidis Acting both as a Repressor and as an Activator of Gene Transcription [O] . Raffaele Ieva, Cristina Alaimo, Isabel Delany, 2005

机译：CrgA是脑膜炎奈瑟氏球菌的诱导型LysR型调节子既可以作为基因转录的阻遏物又可以作为基因转录激活物。

7. CrgA Is an Inducible LysR-Type Regulator of Neisseria meningitidis, Acting both as a Repressor and as an Activator of Gene Transcription [O] . Ieva, Raffaele, Alaimo, Cristina, Delany, Isabel, 2005

机译：CrgA是脑膜炎奈瑟氏球菌的诱导型LysR型调节子，既可以作为基因转录的阻遏物，又可以作为基因转录激活物。

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2. 奈瑟氏脑膜炎球菌铁调蛋白性质的研究 [J] . 尹翠凤 . 微生物学免疫学进展 . 1995,第004期

3. 2013-2015年北京市门头沟区健康人群脑膜炎奈瑟氏球菌带菌率分析 [J] . 刘海涛 ,庄国良 ,王志越 . 首都公共卫生 . 2016,第002期

4. 脑膜炎奈瑟氏球菌感染中毒症1例 [J] . 陶思 ,汤多壮 ,汤屹 . 内科急危重症杂志 . 2008,第003期

5. A群奈瑟氏脑膜炎球菌荚膜多糖-破伤风类毒素结合疫苗的制备及其免疫原性研究 [J] . 刘方蕾 ,吴兵 ,杜琳 . 微生物学免疫学进展 . 2005,第002期

1. 含重组菌毛蛋白的抗淋病奈瑟氏球茵或脑膜炎奈瑟氏球菌的疫苗 [P] . 中国专利： CN1245512C . 2006.03.15

2. 含重组菌毛蛋白的抗淋病奈瑟氏球茵或脑膜炎奈瑟氏球菌的疫苗 [P] . 中国专利： CN1298446A . 2001-06-06

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机译：具有鼠疫奈瑟氏菌抗原活性的肽，编码相同核苷酸的多核苷酸，预防和治疗鼠疫奈瑟氏菌引起的疾病和病症的疫苗，（抗体）与所述肽的抗体偶联

4. A DIAGNOSTIC KIT FOR THE DETECTION AND/OR QUANTIFICATION OF THE NUCLEIC ACIDS OF ANY COMBINATION OF THE MICROBIAL SPECIES AND/OR GENERA SELECTED FROM THE GROUP CONSISTING OF ENTEROCOCCUS FAECIUM, LISTERIA MONOCYTOGENES, NEISSERIA MENINGITIDIS, STAPHYLOCOCCUS SAPROPHYTICUS, STREPTOCOCCUS AGALACTIAE, CANDIDA ALBICANS, ENTEROCOCCUS SPECIES, NEISSERIA SPECIES, STAPHYLOCOCCUS SPECIES, STREPTOCOCCUS SPECIES AND CANDIDA SPECIES. [P] . 外国专利： IN212093B . 2007-11-16

机译：一种用于检测和/或定量微生物物种和/或属的任何组合的核酸的诊断试剂盒，所述微生物选自肠球菌粪便，单核细胞增多性肠炎，念珠菌，脓疱病，葡萄球菌，葡萄球菌物种，奈瑟菌属物种，葡萄球菌物种，链球菌物种和念珠菌物种。

5. A DIAGNOSTIC KIT FOR THE DETECTION AND/OR QUANTIFICATION OF THE NUCLEIC ACIDS OF ANY COMBINATION OF THE MICROBIAL SPECIES AND/OR GENERA SELECTED FROM THE GROUP CONSISTING OF ENTEROCOCCUS FAECIUM, LISTERIA MONOCYTOGENES, NEISSERIA MENINGITIDIS, STAPHYLOCOCCUS SAPROPHYTICUS, STREPTOCOCCUS AGALACTIAE, CANDIDA ALBICANS, ENTEROCOCCUS SPECIES, NEISSERIA SPECIES, STAPHYLOCOCCUS SPECIES, STREPTOCOCCUS SPECIES AND CANDIDA SPECIES. [P] . 外国专利： IN2003KO00020A . 2005-07-01

机译：一种用于检测和/或定量微生物物种和/或属的任何组合的核酸的诊断试剂盒，所述微生物选自肠球菌粪便，利斯特菌单核细胞增多症，奈瑟球菌，脓疱病，葡萄球菌，葡萄球菌物种，奈瑟菌属物种，葡萄球菌物种，链球菌物种和念珠菌物种。

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CrgA Is an Inducible LysR-Type Regulator of Neisseria meningitidis, Acting both as a Repressor and as an Activator of Gene Transcription

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