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首页> 外文期刊>Journal of bacteriology >Structural Characterization of an Oligosaccharide Made by Neisseria sicca
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Structural Characterization of an Oligosaccharide Made by Neisseria sicca

机译：干燥奈瑟菌的寡糖的结构表征

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摘要

Neisseria sicca 4320 expresses two carbohydrate-containing components with sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobilities that resemble those of lipooligosaccharide and lipopolysaccharide. Using matrix-assisted laser desorption ionization—time of flight and electrospray ionization mass spectrometry, we characterized a disaccharide carbohydrate repeating unit expressed by this strain. Gas chromatography identified the sugars composing the unit as rhamnose and N-acetyl-d-glucosamine. Glycosidase digestion confirmed the identity of the nonreducing terminal sugar of the disaccharide and established its β-anomeric configuration. Mass spectrometry analysis and lectin binding were used to verify the linkages within the disaccharide repeat. The results revealed that the disaccharide repeat is [-4) β-l-rhamnose (1-3) β-N-acetyl-d-glucosamine (1-] with an N-acetyl-d-glucosamine nonreducing terminus. This work is the first structural characterization of a molecule that possesses rhamnose in the genus Neisseria.
机译： sicca 4320通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳迁移率表达了两种含脂成分，类似于脂寡糖和脂多糖。使用基质辅助激光解吸电离—飞行时间和电喷雾电离质谱，我们表征了由该菌株表达的二糖碳水化合物重复单元。气相色谱法鉴定了组成单位的糖为鼠李糖和 N -乙酰基-d-葡萄糖胺。糖苷酶消化证实了二糖的非还原性末端糖的身份，并建立了其β-异头构型。质谱分析和凝集素结合用于验证二糖重复序列内的连接。结果显示，二糖重复序列为[-4）β-1鼠李糖（1-3）β- N -乙酰基-d-葡萄糖胺（1-）和 N -乙酰基-d-氨基葡萄糖非还原末端，这项工作是在 Neisseria 属中具有鼠李糖的分子的首次结构表征。

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