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首页> 外文期刊>Journal of bacteriology >Surface-Layer (S-Layer) Proteins Sap and EA1 Govern the Binding of the S-Layer-Associated Protein BslO at the Cell Septa of Bacillus anthracis
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Surface-Layer (S-Layer) Proteins Sap and EA1 Govern the Binding of the S-Layer-Associated Protein BslO at the Cell Septa of Bacillus anthracis

机译：表面层（S层）蛋白Sap和EA1控制炭疽芽孢杆菌细胞隔中S层相关蛋白Bs10的结合。

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The Gram-positive pathogen Bacillus anthracis contains 24 genes whose products harbor the structurally conserved surface-layer (S-layer) homology (SLH) domain. Proteins endowed with the SLH domain associate with the secondary cell wall polysaccharide (SCWP) following secretion. Two such proteins, Sap and EA1, have the unique ability to self-assemble into a paracrystalline layer on the surface of bacilli and form S layers. Other SLH domain proteins can also be found within the S layer and have been designated Bacillus S-layer-associated protein (BSLs). While both S-layer proteins and BSLs bind the same SCWP, their deposition on the cell surface is not random. For example, BslO is targeted to septal peptidoglycan zones, where it catalyzes the separation of daughter cells. Here we show that an insertional lesion in the sap structural gene results in elongated chains of bacilli, as observed with a bslO mutant. The chain length of the sap mutant can be reduced by the addition of purified BslO in the culture medium. This complementation in trans can be explained by an increased deposition of BslO onto the surface of sap mutant bacilli that extends beyond chain septa. Using fluorescence microscopy, we observed that the Sap S layer does not overlap the EA1 S layer and slowly yields to the EA1 S layer in a growth-phase-dependent manner. Although present all over bacilli, Sap S-layer patches are not observed at septa. Thus, we propose that the dynamic Sap/EA1 S-layer coverage of the envelope restricts the deposition of BslO to the SCWP at septal rings.
机译：革兰氏阳性病原体炭疽杆菌包含24个基因，其产物带有结构保守的表面层（S层）同源性（SLH）域。分泌后，具有SLH结构域的蛋白质与次级细胞壁多糖（SCWP）缔合。 Sap和EA1这两种蛋白质具有独特的能力，可以自组装为细菌表面上的顺晶层并形成S层。其他SLH域蛋白也可以在S层中找到，并被称为 B 芽孢杆菌S层相关蛋白（BSLs）。尽管S层蛋白和BSL都结合相同的SCWP，但它们在细胞表面的沉积并不是随机的。例如，Bs10靶向于间隔肽聚糖区域，在该处催化子细胞的分离。在这里，我们显示，在 sap 结构基因中出现的插入病变会导致细菌的细长链，如使用 bslO 突变体所观察到的。可通过在培养基中添加纯化的BslO来减少 sap 突变体的链长。 trans 中的这种互补性可以通过BslO在延伸超出链间隔的 sap 突变杆菌表面上的沉积增加来解释。使用荧光显微镜，我们观察到Sap S层不与EA1 S层重叠，并且以生长相依赖的方式缓慢屈服于EA1 S层。尽管遍布细菌，但在隔垫上未观察到Sap S层斑块。因此，我们提出包膜的动态Sap / EA1 S层覆盖限制了Bs10在隔膜环处向SCWP的沉积。

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