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首页> 外文期刊>Journal of bacteriology >Transcriptomic Analysis Reveals a Bifurcated Terephthalate Degradation Pathway in Rhodococcus sp. Strain RHA1
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Transcriptomic Analysis Reveals a Bifurcated Terephthalate Degradation Pathway in Rhodococcus sp. Strain RHA1

机译：转录组学分析揭示了红球菌属中对苯二甲酸的分叉降解途径。菌株RHA1

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Phthalate isomers and their esters are important pollutants whose biodegradation is not well understood. Rhodococcus sp. strain RHA1 is notable for its ability to degrade a wide range of aromatic compounds. RHA1 was previously shown to degrade phthalate (PTH) and to have genes putatively encoding terephthalate (TPA) degradation. Transcriptomic analysis of 8,213 genes indicated that 150 were up-regulated during growth on PTH and that 521 were up-regulated during growth on TPA. Distinct ring cleavage dioxygenase systems were differentially expressed during growth on PTH and TPA. Genes encoding the protocatechuate (PCA) pathway were induced on both substrates, while genes encoding the catechol branch of the PCA pathway were additionally induced only on TPA. Accordingly, protocatechuate-3,4-dioxygenase activity was induced in cells grown on both substrates, while catechol-1,2-dioxygenase activity was induced only in cells grown on TPA. Knockout analysis indicated that pcaL, encoding 3-oxoadipate enol-lactone hydrolase and 4-carboxymuconolactone decarboxylase, was required for growth on both substrates but that pcaB, encoding β-carboxy-cis,cis-muconate lactonizing enzyme, was required for growth on PTH only. These results indicate that PTH is degraded solely via the PCA pathway, whereas TPA is degraded via a bifurcated pathway that additionally includes the catechol branch of the PCA pathway.
机译：邻苯二甲酸酯异构体及其酯类是重要的污染物，人们对其生物降解尚不十分了解。 红球菌 sp。 RHA1菌株具有降解多种芳香族化合物的能力。以前显示RHA1可降解邻苯二甲酸酯（PTH），并具有假定编码对苯二甲酸酯（TPA）降解的基因。对8,213个基因的转录组分析表明，在PTH上的生长过程中有150个上调，在TPA上的生长过程中有521个上调。不同的环裂解双加氧酶系统在PTH和TPA上生长期间差异表达。在两种底物上均诱导了编码原儿茶酸（PCA）途径的基因，而仅在TPA上另外诱导了编码PCA途径的儿茶酚分支的基因。因此，在两种底物上生长的细胞中均诱导了原儿茶酸-3，4-二加氧酶活性，而仅在TPA上生长的细胞中诱导了儿茶酚-1，2-二加氧酶活性。敲除分析表明， pcaL 编码3-氧代己二酸烯醇内酯水解酶和4-羧基粘康酸内酯脱羧酶，在两种底物上均需要生长，而 pcaB 编码β-羧基-仅在PTH上生长需要顺式，顺式-粘康酸酯内酯化酶。这些结果表明，PTH仅通过PCA途径降解，而TPA通过另外包含PCA途径邻苯二酚分支的分支途径降解。

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