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首页> 外文期刊>Journal of bacteriology >The Xenorhabdus nematophila nilABC Genes Confer the Ability of Xenorhabdus spp. To Colonize Steinernema carpocapsae Nematodes
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The Xenorhabdus nematophila nilABC Genes Confer the Ability of Xenorhabdus spp. To Colonize Steinernema carpocapsae Nematodes

机译:Xenorhabdus nematophila nilABC基因赋予Xenorhabdus spp的能力。殖民Steinernema carpocapsae线虫

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Members of the Steinernema genus of nematodes are colonized mutualistically by members of the Xenorhabdus genus of bacteria. In nature, Steinernema carpocapsae nematodes are always found in association with Xenorhabdus nematophila bacteria. Thus, this interaction, like many microbe-host associations, appears to be species specific. X. nematophila requires the nilA, nilB, and nilC genes to colonize S. carpocapsae. In this work, we showed that of all the Xenorhabdus species examined, only X. nematophila has the nilA, nilB, and nilC genes. By exposing S. carpocapsae to other Xenorhabdus spp., we established that only X. nematophila is able to colonize S. carpocapsae; therefore, the S. carpocapsae-X. nematophila interaction is species specific. Further, we showed that introduction of the nilA, nilB, and nilC genes into other Xenorhabdus species enables them to colonize the same S. carpocapsae host tissue that is normally colonized by X. nematophila. Finally, sequence analysis supported the idea that the nil genes were horizontally acquired. Our findings indicate that a single genetic locus determines host specificity in this bacteria-animal mutualism and that host range expansion can occur through the acquisition of a small genetic element.
机译:线虫的属的成员被细菌的 Xenorhabdus 属的成员相互殖民。在自然界中,总是发现 Steinernema carpocapsae 线虫与 Xenorhabdus nematophila 细菌有关。因此,这种相互作用就像许多微生物与宿主之间的联系一样,似乎是物种特异性的。 X。线虫病需要 nilA nilB nilC 基因来定殖 S。腕果科。在这项工作中,我们显示了检查的所有 Xenorhabdus 物种中,只有 X。嗜线虫病具有 nilA nilB nilC 基因。通过暴露 S。 carpocapsae 到其他 Xenorhabdus spp。,我们确定只有 X。线虫能够在 S上定殖。腕果科;因此, S。腕cap科X.线虫的相互作用是特定物种的。此外,我们表明将 nilA nilB nilC 基因引入其他 Xenorhabdus 物种使他们能够定殖相同的 S。 carcapcapsae 宿主组织通常被X X定殖。线虫。最后,序列分析支持了 nil 基因是水平获取的想法。我们的发现表明,单个遗传基因座决定了这种细菌与动物的共生关系中的宿主特异性,并且宿主范围的扩大可以通过获取一个小的遗传元件而发生。

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