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首页> 外文期刊>Molecular Microbiology >Identification of Xenorhabdus nematophila genes required for mutualistic colonization of Steinernema carpocapsae nematodes.
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Identification of Xenorhabdus nematophila genes required for mutualistic colonization of Steinernema carpocapsae nematodes.

机译：鉴定嗜盐线虫线虫线虫相互定植所需的线虫线虫基因。

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摘要

One stage in the symbiotic interaction between the bacterium Xenorhabdus nematophila and its nematode host, Steinernema carpocapsae, involves the species-specific colonization of the nematode intestinal vesicle by the bacterium. To characterize the bacterial molecular determinants that are essential for vesicle colonization, we adapted and applied a signature-tagged mutagenesis (STM) screen to this system. We identified 15 out of 3000 transposon mutants of X. nematophila with at least a 15-fold reduction in average vesicle colonization. These 15 mutants harbour disruptions in nine separate loci. Three of these loci have predicted open reading frames (ORFs) with similarity to genes (rpoS, rpoE, lrp) encoding regulatory proteins; two have predicted ORFs with similarity to genes (aroA, serC) encoding amino acid biosynthetic enzymes; one, designated nilB (nematode intestine localization), has an ORF with similarity to a gene encoding a putative outer membrane protein (OmpU) in Neisseria; and three, nilA, nilC and nilD, have no apparent homologues in the public database. nilA, nilB and nilC are linked on a single 4 kb locus. nilB and nilC are > 104-fold reduced in their ability to colonize the nematode vesicle and are predicted to encode membrane-localized proteins. The nilD locus contains an extensive repeat region and several small putative ORFs. Other than reduced colonization, the nilB, nilC and nilD mutants did not display alterations in any other phenotype tested, suggesting a specific role for these genes in allowing X. nematophila to associate with the nematode host.
机译：细菌Xenorhabdus nematophila与它的线虫宿主Steinernema carpocapsae之间的共生相互作用的一个阶段涉及该细菌对线虫肠道小泡的物种特异性定殖。为了表征对于囊泡定殖必不可少的细菌分子决定因素，我们对该系统进行了适应性标记诱变（STM）筛选并将其应用于该系统。我们确定了X. nematophila的3000个转座子突变体中的15个，平均囊泡定殖率至少降低了15倍。这15个突变体在9个单独的基因座中具有破坏。这些基因座中的三个已经预测了与编码调控蛋白的基因（rpoS，rpoE，lrp）具有相似性的开放阅读框（ORF）。两项预测的ORF与编码氨基酸生物合成酶的基因（aroA，serC）相似;一个名为nilB（线虫肠道定位）的人，其ORF与编码奈瑟氏菌中假定的外膜蛋白（OmpU）的基因相似。 nilA，nilC和nilD这三个在公共数据库中没有明显的同源性。 nilA，nilB和nilC连接在单个4 kb基因座上。 nilB和nilC在线虫囊泡中的定殖能力降低了104倍以上，并预计将编码膜定位蛋白。 nilD基因座包含一个广泛的重复区域和几个小的推定ORF。除了定居减少外，nilB，nilC和nilD突变体在测试的任何其他表型中均未显示出改变，这暗示了这些基因在允许线虫嗜血杆菌与线虫宿主结合中的特定作用。

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