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首页> 外文期刊>Journal of bacteriology >Genome Sequencing of a Genetically Tractable Pyrococcus furiosus Strain Reveals a Highly Dynamic Genome
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Genome Sequencing of a Genetically Tractable Pyrococcus furiosus Strain Reveals a Highly Dynamic Genome

机译：基因可测序的激烈热球菌菌株的基因组测序揭示了高度动态的基因组。

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The model archaeon Pyrococcus furiosus grows optimally near 100°C on carbohydrates and peptides. Its genome sequence (NCBI) was determined 12 years ago. A genetically tractable strain, COM1, was very recently reported, and here we describe its genome sequence. Of 1,909,827 bp in size, it is 1,571 bp longer (0.1%) than the reference NCBI sequence. The COM1 genome contains numerous chromosomal rearrangements, deletions, and single base changes. COM1 also has 45 full or partial insertion sequences (ISs) compared to 35 in the reference NCBI strain, and these have resulted in the direct deletion or insertional inactivation of 13 genes. Another seven genes were affected by chromosomal deletions and are predicted to be nonfunctional. In addition, the amino acid sequences of another 102 of the 2,134 predicted gene products are different in COM1. These changes potentially impact various cellular functions, including carbohydrate, peptide, and nucleotide metabolism; DNA repair; CRISPR-associated defense; transcriptional regulation; membrane transport; and growth at 72°C. For example, the IS-mediated inactivation of riboflavin synthase in COM1 resulted in a riboflavin requirement for growth. Nevertheless, COM1 grew on cellobiose, malto-oligosaccharides, and peptides in complex and minimal media at 98 and 72°C to the same extent as did both its parent strain and a new culture collection strain (DSMZ 3638). This was in spite of COM1 lacking several metabolic enzymes, including nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase and beta-glucosidase. The P. furiosus genome is therefore of high plasticity, and the availability of the COM1 sequence will be critical for the future studies of this model hyperthermophile.
机译：模型古细菌激烈热球菌在碳水化合物和多肽上可在100°C附近最佳生长。它的基因组序列（NCBI）是12年前确定的。最近报道了一种可遗传控制的菌株COM1，在此我们描述其基因组序列。大小为1,909,827 bp，比参考NCBI序列长1,571 bp（0.1％）。 COM1基因组包含许多染色体重排，缺失和单碱基变化。与参考NCBI菌株中的35个序列相比，COM1还具有45个完整或部分插入序列（IS），这些序列导致13个基因的直接缺失或插入失活。另外七个基因受到染色体缺失的影响，并被认为是无功能的。此外，COM1中2134个预测基因产物中的另外102个氨基酸序列不同。这些变化可能影响各种细胞功能，包括碳水化合物，肽和核苷酸代谢。 DNA修复; CRISPR相关防御;转录调控膜运输并在72°C下生长。例如，COM1中IS介导的核黄素合酶失活导致了核黄素对生长的需求。然而，COM1在复杂的基本培养基中在纤维二糖，麦芽低聚糖和多肽上生长的温度为98和72°C，其亲和菌株和新培养物收集菌株（DSMZ 3638）的生长程度相同。尽管COM1缺少几种代谢酶，包括非磷酸化的3-磷酸甘油醛脱氢酶和β-葡萄糖苷酶。因此，P。furiosus基因组具有很高的可塑性，COM1序列的可用性对于该模型嗜热菌的未来研究至关重要。

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