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首页> 外文期刊>Journal of bacteriology >In Vivo Interactions between Toxin-Antitoxin Proteins Epsilon and Zeta of Streptococcal Plasmid pSM19035 in Saccharomyces cerevisiae
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In Vivo Interactions between Toxin-Antitoxin Proteins Epsilon and Zeta of Streptococcal Plasmid pSM19035 in Saccharomyces cerevisiae

机译：酿酒酵母中毒素-抗毒素蛋白Epsilon和链球菌质粒pSM19035的Zeta的体内相互作用。

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摘要

The widespread prokaryotic toxin-antitoxin (TA) systems involve conditional interaction between two TA proteins. The interaction between the Epsilon and Zeta proteins, constituting the TA system of plasmid pSM19035 from Streptococcus pyogenes, was detected in vivo using a yeast two-hybrid system. As we showed using Saccharomyces cerevisiae, the Zeta toxin hybrid gene also exerts its toxic effects in a dose-dependent manner in eukaryotic cells. Analysis of mutant proteins in the two-hybrid system demonstrated that the N-terminal part of Zeta and the N-terminal region of Epsilon are involved in the interaction. The N-terminal region of the Zeta protein and its ATP/GTP binding motif were found to be responsible for the toxicity.
机译：广泛存在的原核毒素-抗毒素（TA）系统涉及两个TA蛋白之间的条件相互作用。利用酵母双杂交系统在体内检测到Epsilon和Zeta蛋白之间的相互作用，构成了化脓链球菌质粒pSM19035的TA系统。正如我们使用酿酒酵母所显示的，Zeta毒素杂种基因在真核细胞中也以剂量依赖的方式发挥其毒性作用。对双杂交系统中突变蛋白的分析表明，Zeta的N末端部分和Epsilon的N末端区域参与了相互作用。发现Zeta蛋白的N端区域及其ATP / GTP结合基序是造成毒性的原因。

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