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首页> 外文期刊>Journal of bacteriology >tISCpe8, an IS1595-Family Lincomycin Resistance Element Located on a Conjugative Plasmid in Clostridium perfringens
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tISCpe8, an IS1595-Family Lincomycin Resistance Element Located on a Conjugative Plasmid in Clostridium perfringens

机译：tISCpe8，IS1595-家族对林可霉素的抗性元件，位于产气荚膜梭菌的结合质粒上

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Clostridium perfringens is a normal gastrointestinal organism that is a reservoir for antibiotic resistance genes and can potentially act as a source from which mobile elements and their associated resistance determinants can be transferred to other bacterial pathogens. Lincomycin resistance in C. perfringens is common and is usually encoded by erm genes that confer macrolide-lincosamide-streptogramin B resistance. In this study we identified strains that are lincomycin resistant but erythromycin sensitive and showed that the lincomycin resistance determinant was plasmid borne and could be transferred to other C. perfringens isolates by conjugation. The plasmid, pJIR2774, is the first conjugative C. perfringens R-plasmid to be identified that does not confer tetracycline resistance. Further analysis showed that resistance was encoded by the lnuP gene, which encoded a putative lincosamide nucleotidyltransferase and was located on tISCpe8, a functional transposable genetic element that was a member of the IS1595 family of transposon-like insertion sequences. This element had significant similarity to the mobilizable lincomycin resistance element tISSag10 from Streptococcus agalactiae. Like tISSag10, tISCpe8 carries a functional origin of transfer within the resistance gene, allowing the element to be mobilized by the conjugative transposon Tn916. The similarity of these elements and the finding that they both contain an oriT-like region support the hypothesis that conjugation may result in the movement of DNA modules that are not obviously mobile since they are not linked to conjugation or mobilization functions. This process likely plays a significant role in bacterial adaptation and evolution.
机译：产气荚膜梭菌是一种正常的胃肠道生物，它是抗生素抗性基因的储存库，并有可能作为一种来源，可将移动元素及其相关的抗性决定因子从中转移到其他细菌病原体中。 C对林可霉素的耐药性。产气荚膜菌很常见，通常由赋予大环内酯类-林可酰胺-链霉菌素B耐药性的 erm 基因编码。在这项研究中，我们鉴定了对林可霉素具有抗性但对红霉素敏感的菌株，并表明该林可霉素抗性决定簇是质粒携带的，并且可以转移至其他 C。产气荚膜菌通过缀合分离。质粒pJIR2774是第一个缀合的C。鉴定出不赋予四环素抗性的产气荚膜杆菌R质粒。进一步的分析表明，抗性由 lnuP 基因编码，该基因编码一种假定的林可酰胺酰胺基核苷酸转移酶，位于tIS Cpe8 上，tIS Cpe8 是该功能的转座遗传元件，是IS 1595 系列转座子样插入序列。该元素与无乳链球菌的可移动的林可霉素抗性元件tIS Sag10 相似。与tIS Sag10 一样，tIS Cpe8 在抗性基因内也具有转移的功能性起源，从而使该元件能够通过结合转座子Tn 916 动员。 。这些元素的相似性以及它们都包含一个 oriT 样区域的发现，支持了这样的假设，即结合可能导致DNA模块的运动，而这些模块显然不是可移动的，因为它们与结合或连接没有联系。动员功能。这个过程可能在细菌适应和进化中起重要作用。

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