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首页> 外文期刊>Journal of bacteriology >Characterization of IsaA and SceD, Two Putative Lytic Transglycosylases of Staphylococcus aureus
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Characterization of IsaA and SceD, Two Putative Lytic Transglycosylases of Staphylococcus aureus

机译：IsaA和SceD，金黄色葡萄球菌的两个假定的裂解转糖基酶的表征

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摘要

Bacterial cell wall peptidoglycan is a dynamic structure requiring hydrolysis to allow cell wall growth and division. Staphylococcus aureus has many known and putative peptidoglycan hydrolases, including two likely lytic transglycosylases. These two proteins, IsaA and SceD, were both found to have autolytic activity. Regulatory studies showed that the isaA and sceD genes are partially mutually compensatory and that the production of SceD is upregulated in an isaA mutant. The expression of sceD is also greatly upregulated by the presence of NaCl. Several regulators of isaA and sceD expression were identified. Inactivation of sceD resulted in impaired cell separation, as shown by light microscopy, and “clumping” of bacterial cultures. An isaA sceD mutant is attenuated for virulence, while SceD is essential for nasal colonization in cotton rats, thus demonstrating the importance of cell wall dynamics in host-pathogen interactions.
机译：细菌细胞壁肽聚糖是一种动态结构，需要水解才能使细胞壁生长和分裂。 金黄色葡萄球菌有许多已知的和推定的肽聚糖水解酶，包括两种可能的裂解转糖基酶。发现这两种蛋白质，IsaA和SceD，都具有自溶活性。监管研究表明， isaA 和 sceD 基因是部分相互补偿的，并且在 isaA 突变体中SceD的产生上调。 NaCl的存在还大大上调了 sceD 的表达。确定了几个 isaA 和 sceD 表达的调节子。如光学显微镜所示， sceD 的失活导致细胞分离受损，并使细菌培养物“结块”。一个 isaA sceD 突变体的毒力减弱，而SceD对棉鼠鼻内定殖至关重要，因此证明了细胞壁动力学在宿主-病原体相互作用中的重要性。

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