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首页> 外文期刊>Journal of bacteriology >A Distant Homologue of the FlgT Protein Interacts with MotB and FliL and Is Essential for Flagellar Rotation in Rhodobacter sphaeroides
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A Distant Homologue of the FlgT Protein Interacts with MotB and FliL and Is Essential for Flagellar Rotation in Rhodobacter sphaeroides

机译：FlgT蛋白的遥远同源物与MotB和FliL相互作用，对于球形红球菌鞭毛旋转至关重要。

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In this work, we describe a periplasmic protein that is essential for flagellar rotation in Rhodobacter sphaeroides. This protein is encoded upstream of flgA, and its expression is dependent on the flagellar master regulator FleQ and on the class III flagellar activator FleT. Sequence comparisons suggest that this protein is a distant homologue of FlgT. We show evidence that in R. sphaeroides, FlgT interacts with the periplasmic regions of MotB and FliL and with the flagellar protein MotF, which was recently characterized as a membrane component of the flagellum in this bacterium. In addition, the localization of green fluorescent protein (GFP)-MotF is completely dependent on FlgT. The Mot? phenotype of flgT cells was weakly suppressed by point mutants of MotB that presumably keep the proton channel open and efficiently suppress the Mot? phenotype of motF and fliL cells, indicating that FlgT could play an additional role beyond the opening of the proton channel. The presence of FlgT in purified filament-hook-basal bodies of the wild-type strain was confirmed by Western blotting, and the observation of these structures under an electron microscope showed that the basal bodies from flgT cells had lost the ring that covers the LP ring in the wild-type structure. Moreover, MotF was detected by immunoblotting in the basal bodies obtained from the wild-type strain but not from flgT cells. From these results, we suggest that FlgT forms a ring around the LP ring, which anchors MotF and stabilizes the stator complex of the flagellar motor.
机译：在这项工作中，我们描述了一种球形蛋白，对于球形球形红细菌中鞭毛旋转至关重要。该蛋白在 flgA 的上游编码，其表达取决于鞭毛主调控因子FleQ和III类鞭毛激活因子FleT。序列比较表明该蛋白是FlgT的遥远同源物。我们显示出证据，在球形红球菌中，FlgT与MotB和FliL的周质区域相互作用，并与鞭毛蛋白MotF相互作用，该蛋白最近被表征为该细菌中鞭毛的膜成分。此外，绿色荧光蛋白（GFP）-MotF的定位完全取决于FlgT。 MotB的点突变体弱抑制了 flgT 细胞的Mot ？表型，该突变体可能保持质子通道开放并有效地抑制了Mot ？表型的 motF 和 fliL 细胞，表明FlgT可能在质子通道开放之外发挥额外的作用。通过Western印迹证实野生型菌株的纯化的丝钩基体中存在FlgT，并且在电子显微镜下观察这些结构表明， flgT 细胞的基体具有在野生型结构中丢失了覆盖LP环的环。此外，通过免疫印迹检测了来自野生型菌株而不是 flgT 细胞的基体中的MotF。根据这些结果，我们建议FlgT在LP环周围形成一个环，该环锚固MotF并稳定鞭毛马达的定子复合体。

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