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首页> 外文期刊>Journal of bacteriology >Inactivation of σE and σG in Clostridium acetobutylicum Illuminates Their Roles in Clostridial-Cell-Form Biogenesis, Granulose Synthesis, Solventogenesis, and Spore Morphogenesis
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Inactivation of σE and σG in Clostridium acetobutylicum Illuminates Their Roles in Clostridial-Cell-Form Biogenesis, Granulose Synthesis, Solventogenesis, and Spore Morphogenesis

机译：丙酮丁醇梭菌中σE和σG的失活阐明了它们在梭菌细胞形式的生物发生，颗粒合成，溶剂形成和孢子形态发生中的作用。

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摘要

Central to all clostridia is the orchestration of endospore formation (i.e., sporulation) and, specifically, the roles of differentiation-associated sigma factors. Moreover, there is considerable applied interest in understanding the roles of these sigma factors in other stationary-phase phenomena, such as solvent production (i.e., solventogenesis). Here we separately inactivated by gene disruption the major sporulation-specific sigma factors, σE and σG, and performed an initial analysis to elucidate their roles in sporulation-related morphogenesis and solventogenesis in Clostridium acetobutylicum. The terminal differentiation phenotype for the sigE inactivation mutant stalled in sporulation prior to asymmetric septum formation, appeared vegetative-like often with an accumulation of DNA at both poles, frequently exhibited two longitudinal internal membranes, and did not synthesize granulose. The sigE inactivation mutant did produce the characteristic solvents (i.e., butanol and acetone), but the extent of solventogenesis was dependent on the physiological state of the inoculum. The sigG inactivation mutant stalled in sporulation during endospore maturation, exhibiting engulfment and partial cortex and spore coat formation. Lastly, the sigG inactivation mutant did produce granulose and exhibited wild-type-like solventogenesis.
机译：所有梭状芽胞杆菌的中心是编配内生孢子（即孢子形成），尤其是分化相关的西格玛因子的作用。此外，在理解这些σ因子在其他固定相现象，例如溶剂产生（即，溶剂生成）中的作用方面具有相当大的应用兴趣。在这里，我们通过基因破坏分别灭活了主要的孢子特异性σ因子σ E 和σ G ，并进行了初步分析以阐明它们在孢子相关的形态发生和发育中的作用。丙酮丁醇梭菌中的溶剂生成。 sigE 失活突变体的终末分化表型在不对称隔膜形成之前在孢子形成中停滞，表现为植物性，通常在两个极都具有DNA积累，经常表现出两个纵向内膜，并且不合成颗粒状的 sigE 失活突变体确实产生了特征性的溶剂（即丁醇和丙酮），但是溶剂生成的程度取决于接种物的生理状态。 sigG 失活突变体在孢子成熟过程中停滞在孢子形成中，表现出吞噬，部分皮质和孢子被膜形成。最后， sigG 失活突变体的确产生了颗粒，并表现出类似野生型的溶剂生成。

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