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Irradiance and duration dependence of the cone photoreceptor intrinsic reflectance response

机译:锥光感受器本征反射响应的辐照度和持续时间依赖性

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Adaptive optics (AO) imaging of the human cone mosaic has shown that a visible stimulus can induce intrinsic changes in cone infrared reflectivity. We studied this intrinsic response as a function of stimulus irradiance and duration. Five subjects were imaged using a previously described AO scanning light ophthalmoscope. Following 2 minutes of dark adaptation, image sequences were obtained 0.65?° from the fovea using a 790 nm imaging light. Four seconds after the start of each sequence, a 680 nm stimulus of variable irradiance (0.037 to 18.9 ??W/deg^2) and duration (0.2 to 2 secs) was delivered to one half of the imaging field. Reflectance signals were extracted from each cone and standardized to their pre-stimulus values. The difference in the standard deviation of the reflectance of stimulated and control (unstimulated) cones was determined at each time point. The peak was extracted from the resultant time-varying response. Peak response increased with both stimulus irradiance and duration, qualitatively consistent with a dependence of the response on photoreceptor function.
机译:人体视锥马赛克的自适应光学(AO)成像显示,可见刺激可以诱发视锥红外反射率的固有变化。我们研究了这种内在响应作为刺激辐照度和持续时间的函数。使用先前描述的AO扫描光检眼镜对五个对象成像。暗适应2分钟后,使用790 nm成像光从中央凹处获得0.65?°的图像序列。在每个序列开始后四秒钟,将可变辐照度(0.037至18.9ΩW/ deg ^ 2)和持续时间(0.2至2秒)的680 nm刺激传递到成像场的一半。从每个锥体中提取反射信号,并将其标准化为刺激前的值。在每个时间点确定受激和对照(未受激)锥的反射率标准偏差的差异。从所得的时变响应中提取峰。峰值响应随着刺激辐照度和持续时间的增加而增加,在质量上与响应对感光器功能的依赖性一致。

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