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首页> 外文期刊>Scientific reports. >Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology
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Improved antifouling properties and selective biofunctionalization of stainless steel by employing heterobifunctional silane-polyethylene glycol overlayers and avidin-biotin technology

机译：通过使用异双功能硅烷-聚乙二醇覆盖剂和抗生物素蛋白-生物素技术提高不锈钢的防污性能和选择性生物功能

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摘要

A straightforward solution-based method to modify the biofunctionality of stainless steel (SS) using heterobifunctional silane-polyethylene glycol (silane-PEG) overlayers is reported. Reduced nonspecific biofouling of both proteins and bacteria onto SS and further selective biofunctionalization of the modified surface were achieved. According to photoelectron spectroscopy analyses, the silane-PEGs formed less than 10?? thick overlayers with close to 90% surface coverage and reproducible chemical compositions. Consequently, the surfaces also became more hydrophilic, and the observed non-specific biofouling of proteins was reduced by approximately 70%. In addition, the attachment of E. coli was reduced by more than 65%. Moreover, the potential of the overlayer to be further modified was demonstrated by successfully coupling biotinylated alkaline phosphatase (bAP) to a silane-PEG-biotin overlayer via avidin-biotin bridges. The activity of the immobilized enzyme was shown to be well preserved without compromising the achieved antifouling properties. Overall, the simple solution-based approach enables the tailoring of SS to enhance its activity for biomedical and biotechnological applications.
机译：报道了一种基于溶液的简单方法，该方法使用杂双功能硅烷-聚乙二醇（硅烷-PEG）覆盖层来改变不锈钢（SS）的生物功能。减少了蛋白质和细菌对SS的非特异性生物结垢，并实现了改性表面的进一步选择性生物功能化。根据光电子能谱分析，形成的硅烷-PEG少于10-6个。厚的覆盖层，表面覆盖率接近90％，化学成分可重现。因此，表面也变得更加亲水，观察到的蛋白质的非特异性生物结垢减少了约70％。另外，大肠杆菌的附着减少了65％以上。此外，通过生物素化的碱性磷酸酶（bAP）通过亲和素-生物素桥成功地偶联生物素化的碱性磷酸酶（bAP）与硅烷-PEG-生物素覆盖层，证明了该被修饰层的潜力。固定化酶的活性显示出很好的保存效果，而不会损害获得的防污性能。总体而言，基于解决方案的简单方法可以定制SS，以增强其在生物医学和生物技术应用中的活动。

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