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首页> 外文期刊>Scientific reports. >In vitro Chondrocyte Responses in Mg-doped Wollastonite/Hydrogel Composite Scaffolds for Osteochondral Interface Regeneration
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In vitro Chondrocyte Responses in Mg-doped Wollastonite/Hydrogel Composite Scaffolds for Osteochondral Interface Regeneration

机译：镁掺杂硅灰石/水凝胶复合支架中软骨细胞界面再生的体外软骨细胞反应。

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The zone of calcified cartilage (ZCC) is the mineralized region between the hyaline cartilage and subchondral bone and is critical in cartilage repair. A new non-stoichiometric calcium silicate (10% Ca substituted by Mg; CSi-Mg10) has been demonstrated to be highly bioactive in an osteogenic environment in vivo. This study is aimed to systematically evaluate the potential to regenerate osteochondral interface with different amount of Ca-Mg silicate in hydrogel-based scaffolds, and to compare with the scaffolds containing conventional Ca-phosphate biomaterials. Hydrogel-based porous scaffolds combined with 0–6% CSi-Mg10, 6% β-tricalcium phosphate (β-TCP) or 6% nanohydroxyapatite (nHAp) were made with three-dimensional (3D) printing. An increase in CSi-Mg10 content is desirable for promoting the hypertrophy and mineralization of chondrocytes, as well as cell proliferation and matrix deposition. Osteogenic and chondrogenic induction were both up-regulated in a dose-dependent manner. In comparison with the scaffolds containing 6% β-TCP or nHAp, human deep zone chondrocytes (hDZCs) seeded on CSi-Mg10 scaffold of equivalent concentration exhibited higher mineralization. It is noteworthy that the hDZCs in the 6% CSi-Mg10 scaffolds maintained a higher expression of the calcified cartilage zone specific extracellular matrix marker and hypertrophic marker, collagen type X. Immunohistochemical and Alizarin Red staining reconfirmed these findings. The study demonstrated that hydrogel-based hybrid scaffolds containing 6% CSi-Mg10 are particularly desirable for inducing the formation of calcified cartilage.
机译：钙化软骨区（ZCC）是透明软骨和软骨下骨之间的矿化区域，在软骨修复中至关重要。一种新型非化学计量的硅酸钙（10％Ca被Mg取代; CSi-Mg10）在体内成骨环境中具有很高的生物活性。这项研究旨在系统地评估在水凝胶基支架中用不同量的Ca-Mg硅酸盐再生骨软骨界面的潜力，并与含有常规Ca-磷酸盐生物材料的支架进行比较。通过三维（3D）打印制得了结合0-6％CSi-Mg10、6％β-磷酸三钙（β-TCP）或6％纳米羟基磷灰石（nHAp）的水凝胶基多孔支架。 CSi-Mg10含量的增加对于促进软骨细胞的肥大和矿化以及细胞增殖和基质沉积是理想的。成骨和软骨形成诱导均以剂量依赖性方式上调。与含有6％β-TCP或nHAp的支架相比，接种在等效浓度的CSi-Mg10支架上的人深区软骨细胞（hDZC）表现出更高的矿化度。值得注意的是，在6％CSi-Mg10支架中的hDZCs维持了钙化软骨区特异的细胞外基质标志物和肥大标志物（X型胶原）的更高表达。免疫组织化学和茜素红染色再次证实了这些发现。研究表明，含有6％CSi-Mg10的水凝胶基杂合支架特别适合诱导钙化软骨的形成。

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