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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Antimicrobial efficacy and wound-healing property of a topical ointment containing nitric-oxide-loaded zeolites
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Antimicrobial efficacy and wound-healing property of a topical ointment containing nitric-oxide-loaded zeolites

机译：含有一氧化氮沸石的局部软膏的抗菌疗效和伤口愈合性能

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Topical delivery of nitric oxide (NO) through a wound dressing has the potential to reduce wound infections and improve healing of acute and chronic wounds. This study characterized the antibacterial efficacy of an ointment containing NO-loaded, zinc-exchanged zeolite A that releases NO upon contact with water. The release rate of NO from the ointment was measured using a chemiluminescence detection system. Minimum bactericidal concentration assays were performed using five common wound pathogens, including Gram-negative bacteria (Escherichia coli and Acinetobacter baumannii), Gram-positive bacteria (Staphylococcus epidermidis and meticillin-resistant Staphylococcus aureus) and a fungus (Candida albicans). The time dependence of antimicrobial activity was characterized by performing log-reduction assays at four time points after 1–8 h ointment exposure. The cytotoxicity of the ointment after 24 h was assessed using cultured 3T3 fibroblast cells. Minimum microbicidal concentrations (MMCs) for bacterial organisms (5×107 c.f.u.) ranged from 50 to 100 mg ointment (ml media)?1; the MMC for C. albicans (5×104 c.f.u.) was 50 mg ointment (ml media)?1. Five to eight log reductions in bacterial viability and three log reductions in fungal viability were observed after 8 h exposure to NO–zeolite ointment compared with untreated organisms. Fibroblasts remained viable after 24 h exposure to the same concentration of NO–zeolite ointment as was used in antimicrobial tests. In parallel studies, full-thickness cutaneous wounds on Zucker obese rats healed faster than wounds treated with a control ointment. These data indicate that ointment containing NO-loaded zeolites could potentially be used as a broad-spectrum antimicrobial wound-healing dressing.
机译：通过伤口敷料的一氧化氮（NO）的局部递送有可能降低伤口感染并改善急性和慢性伤口的愈合。本研究表征了含有无负载锌交换沸石A的软膏的抗菌效果，其在与水接触时释放。使用化学发光检测系统测量NO来自软膏的释放速率。使用五种常见的伤口病原体进行最小杀菌浓度测定，包括革兰氏阴性细菌（大肠杆菌和肺杆菌），革氏阳性细菌（葡萄球菌癫痫和型葡萄球菌葡萄球菌）和真菌（念珠菌蛋白质）。抗菌活性的时间依赖性的特征在于在1-8小时后暴露后在四个时间点进行逻辑降低测定。使用培养的3T3成纤维细胞评估24小时后软膏的细胞毒性。用于细菌生物的最小杀微生物浓度（MMC）（5×107℃。）范围为50至100mg软膏（ml培养基）α1;用于C. albicans（5×104 c.u.u.u.）的MMC为50mg软膏（ml培养基）？1。与未处理的生物相比，在8小时内暴露于无沸石软膏后，观察到细菌活力的五到八个核减少，并且在没有治疗的生物的情况下，观察到无沸石软膏后的真菌活力。 24小时暴露于与抗微生物检验中使用的相同浓度的无沸石软膏后，成纤维细胞保持不变。在平行研究中，Zucker肥胖大鼠的全厚皮肤伤口比用对照软膏治疗的伤口愈合得更快。这些数据表明，含有无负载沸石的软膏可能被用作广谱抗菌伤口愈合敷料。

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