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首页> 外文期刊>Journal of Medical Microbiology: An Official Journal of the Pathological Society of Great Britain and Ireland >Characterization of Staphylococcus epidermidis and Staphyloccocus warneri small-colony variants associated with prosthetic-joint infections
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Characterization of Staphylococcus epidermidis and Staphyloccocus warneri small-colony variants associated with prosthetic-joint infections

机译：表征葡萄球菌表征和葡萄球菌的葡萄球菌与假性关节感染相关的小菌落变体

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摘要

We determined the frequency of isolation of staphylococcal small-colony variants (SCVs) from 31 culture-positive patients undergoing revision of total hip prosthesis for aseptic loosening or presumed prosthetic-joint infection (PJI). We analysed auxotrophy of cultured SCVs, their antimicrobial susceptibility profiles and their biofilm-forming capacity. Eight SCV strains were cultivated from six (19 %) patients. All SCVs were coagulase-negative staphylococci (CNS) with Staphylococcus epidermidis as the predominant species; there was also one Staphylococcus warneri SCV. The SCVs were auxotrophic for haemin, with one strain additionally auxotrophic for menadione. We noted the presence of two phenotypically (differences concerning antimicrobial susceptibility) and genetically distinct SCV strains in one patient, as well as the growth of two genetically related SCVs that differed in terms of their morphology and the type of auxotrophy in another. Seven out of eight SCVs were resistant to meticillin and gentamicin. In addition, antibiotic sensitivity testing revealed three multidrug-resistant SCV–normal-morphology isolate pairs. One S. epidermidis SCV harboured icaADBC genes and was found to be a proficient biofilm producer. This paper highlights the involvement of CNS SCVs in the aetiology of PJIs, including what is believed to be the first report of a S. warneri SCV. These subpopulations must be actively sought in the routine diagnosis of implant-associated infections. Moreover, in view of the phenotypic and genetic diversity of some SCV pairs, particular attention should be paid to the investigation of all types of observed colony morphologies, and isolates should be subjected to antimicrobial susceptibility testing.
机译：我们确定了从31种培养阳性患者接受修订的31例培养阳性患者的葡萄球菌小菌落变体（SCV）的频率，用于无菌松动或假定的假体关节感染（PJI）。我们分析了培养的SCV的氛围，其抗微生物敏感性和它们的生物膜形成能力。六（19％）患者培养了八种SCV菌株。所有SCV都是凝壳酶阴性葡萄球菌（CNS），其具有葡萄球菌表皮，作为主要物种;还有一种葡萄球菌SCV。 SCVS是血液中的肺促进营养营养性，一种菌株另外用于植物营养营养营养症。我们注意到在一个患者中存在两种表型（患有抗菌易感性的差异）和遗传上不同的SCV菌株，以及两种遗传相关的SCV的生长，其在其形态和另一种营养营养类型方面不同。八个SCV中的七种对Meticillin和庆大霉素造成耐药。此外，抗生素敏感性测试揭示了三种多药抗性SCV-正常形态学分离对。艾德伯德斯SCV患有ICAADBC基因，发现是熟练的生物膜生产商。本文突出了CNS SCVS在PJI的审查中的参与，包括被认为是S. Warneri SCV的第一个报告。必须在植入物相关感染的常规诊断中积极寻求这些亚步骤。此外，鉴于某些SCV对的表型和遗传多样性，应特别注意对所有类型观察到的菌落形态的调查，并且应进行分离株抗菌易感测试。

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