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首页> 外文期刊>Journal of Clinical Microbiology >Optimization of a rapid test by using fluorescein-conjugated monoclonal antibodies for detection of Chlamydia trachomatis in clinical specimens.
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Optimization of a rapid test by using fluorescein-conjugated monoclonal antibodies for detection of Chlamydia trachomatis in clinical specimens.

机译：通过使用荧光素 - 缀合的单克隆抗体进行快速测试，以检测临床标本中的衣原体衣原体。

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A mixture of two fluorescein isothiocyanate-conjugated monoclonal antibodies (MAbs) was used to optimize a direct specimen test (Chlamydia Direct Specimen Test IF; Clonatec, Paris, France) for detection of chlamydial elementary bodies in clinical specimens. One MAb reacted with a subspecies-specific epitope of the major outer membrane protein (molecular weight 43,000) of Chlamydia trachomatis, whereas the other reacted with the periodate-sensitive genus-specific antigen (molecular weight 11,000) of Chlamydia spp. Nonfat dry milk was the most efficient additive at suppressing the fluorescent background and was included in the antibody preparation. Fc-dependent binding of fluorescein-conjugated MAbs to protein A-containing Staphylococcus aureus was inhibited by addition of purified rabbit immunoglobulin. The Chlamydia Direct Specimen Test IF was compared with tissue culture isolation by using 309 genital specimens. The sensitivity and specificity were 77.4 and 98%, respectively.
机译：使用两种荧光素异硫氰酸酯 - 缀合的单克隆抗体（MAB）的混合物来优化直接试样试验（衣原体直接标本试验，如果ClonaTec，Paris，France）用于检测临床标本中的衣原体小学体。一个MAB与衣原体衣原体的主要外膜蛋白（分子量43,000）的亚种特异性表位反应，而另一个是与衣原体SPP的骨敏感性特异性抗原（分子量11,000）反应。非比特干乳是抑制荧光背景中最有效的添加剂，并包括在抗体制剂中。通过加入纯化的兔免疫球蛋白抑制了荧光素缀合的MAb对含含蛋白α葡萄球菌的Fc依赖性结合。通过使用309生殖器标本将衣原体直接标本测试与组织培养分离进行比较。敏感性和特异性分别为77.4和98％。

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