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首页> 外文期刊>Journal of bacteriology >A Developmentally Regulated Gene Cluster Involved in Conidial Pigment Biosynthesis in Aspergillus fumigatus
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A Developmentally Regulated Gene Cluster Involved in Conidial Pigment Biosynthesis in Aspergillus fumigatus

机译：Aspergillus fumigatus中的共生色素生物合成涉及共生色素生物合成的发育调节基因簇

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Aspergillus fumigatus, a filamentous fungus producing bluish-green conidia, is an important opportunistic pathogen that primarily affects immunocompromised patients. Conidial pigmentation ofA. fumigatus significantly influences its virulence in a murine model. In the present study, six genes, forming a gene cluster spanning 19 kb, were identified as involved in conidial pigment biosynthesis in A. fumigatus. Northern blot analyses showed the six genes to be developmentally regulated and expressed during conidiation. The gene products of alb1 (for “albino 1”), arp1 (for “aspergillus reddish-pink 1”), andarp2 have high similarity to polyketide synthases, scytalone dehydratases, and hydroxynaphthalene reductases, respectively, found in the dihydroxynaphthalene (DHN)-melanin pathway of brown and black fungi. The abr1 gene (for “aspergillus brown 1”) encodes a putative protein possessing two signatures of multicopper oxidases. The abr2 gene product has homology to the laccase encoded by the yA gene of Aspergillus nidulans. The function of ayg1 (for “aspergillus yellowish-green 1”) remains unknown. Involvement of the six genes in conidial pigmentation was confirmed by the altered conidial color phenotypes that resulted from disruption of each gene in A. fumigatus. The presence of a DHN-melanin pathway in A. fumigatus was supported by the accumulation of scytalone and flaviolin in the arp1 deletant, whereas only flaviolin was accumulated in the arp2 deletants. Scytalone and flaviolin are well-known signature metabolites of the DHN-melanin pathway. Based on DNA sequence similarity, gene disruption results, and biochemical analyses, we conclude that the 19-kb DNA fragment contains a six-gene cluster which is required for conidial pigment biosynthesis in A. fumigatus. However, the presence of abr1,abr2, and ayg1 in addition to alb1,arp1, and arp2 suggests that conidial pigment biosynthesis in A. fumigatus is more complex than the known DHN-melanin pathway.
机译： aspergillus fumigatus ，一种产生蓝绿色的丝状真菌，是一种重要的机会主义病原体，主要影响免疫疗效。分析色素沉着 a。 Fumigatus 显着影响其鼠模型的毒力。在本研究中，六个基因形成跨越19 kB的基因簇，被鉴定为在 a中的共分枝颜料生物合成中涉及。 Fumigatus 。 Northern印迹分析显示六个基因在共复合期间发育调节和表达。 ALB1 （用于“白化1”）， ARP1 （用于“曲霉菌红粉红色1”）和 ARP2 具有高相似性分别与聚酮合成酶，锡酮脱水酶和羟基萘还原酶中的棕色和黑色真菌的二羟基萘（DHN）-Melanin途径中发现。 abr1 基因（用于“aspergillus brown 1”）编码具有多个氧化酶的两个特征的推定蛋白质。 abr2 基因产物对由 aspergillus nidulans 的 ya 基因编码的漆酶同源性。 Ayg1 （用于“曲霉黄绿色1”）的功能仍然未知。通过破坏每个基因在 A中断的改变的分枝颜色表型证实了六种基因的参与。 Fumigatus 。在 a中存在Dhn-黑色素途径。 Fumigatus 得到了在 ARP1 删除中的菌硝基酮和Flaviolin的积累支持，而只有Flaviolin被积累在 ARP2 脱血剂中。 Scytalone和Flaviolin是DHN-黑色素途径的众所周知的签名代谢物。基于DNA序列相似性，基因破坏结果和生物化学分析，我们得出结论，19-KB DNA片段含有六基因簇，其在 a中的共生色素生物合成所需的六基因簇。 Fumigatus 。但是，除了 ALB1 之外， ABR1 ， ABR2 ， AYG1 。 ARP1 和 ARP2 表明在 A中的共分子颜料生物合成。 Fumigatus 比已知的DHN-黑色素途径更复杂。

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