• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Uptake and killing of Lyme disease and relapsing fever borreliae in the perfused rat liver and by isolated Kupffer cells.
【24h】

Uptake and killing of Lyme disease and relapsing fever borreliae in the perfused rat liver and by isolated Kupffer cells.

机译：肝脏疾病的摄取与杀伤灌注大鼠肝脏中的发热障碍，孤立的Kupffer细胞。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In situ-perfused rat livers were infused with a single dose of 1.5 x 10(7) radiolabeled borreliae. Significant (P < 0.00005) differences in the liver uptake of the agents of Lyme borreliosis, Borrelia burgdorferi IRS, Borrelia afzelii VS461, and Borrelia garinii PBi, and that of the agents of relapsing fever, Borrelia hermsii, Borrelia parkeri, and Borrelia turicatae, were observed. The liver uptakes ranged between 65.9% for B. burgdorferi IRS and 40.5% for B. turicatae. Neither relapsing fever nor Lyme disease borreliae were recovered from infected livers when the livers were cultured in Barbour-Stoenner-Kelly II medium. The in vitro uptake of B. burgdorferi IRS by isolated rat Kupffer cells was rapid, and within 30 min of the infection, large intracellular aggregates of amorphous material were detectable by immunofluorescence with specific anti-B. burgdorferi antibody. The reculturing of B. burgdorferi IRS from Kupffer cells incubated for 24 h in RPMI medium before inoculation with bacteria was negative. The results obtained in this study indicated that borreliae are efficiently taken up and killed by rat hepatic macrophages in the absence of serum factors.
机译：在原位 - 灌注的大鼠肝脏中，用一剂为1.5×10（7）个放射性标记的博罗纹。显着（p <0.00005）肝脏肝脏肝脏，Borrelia Burgdoriferi，Borrelia Afzelii Vs461和Borrelia Garinii Pbi的差异，以及复发发烧，Borrelia Hermsii，Borrelia Parkeri和Borrelia Turicatae的药物被观察到。 B.Burgdorferi IRS的肝脏增值范围在65.9％之间，B. Turicatae的40.5％。当肝脏在Barbour-Stoenner-Kelly II培养基中培养时，既不从受感染的肝脏中回收复发发烧和莱姆疾病博尔塞雷。 B. Burgdorferi IRS对分离的大鼠Kupffer细胞的体外摄取是快速的，并且在感染后30分钟内，通过具有特异性抗B的免疫荧光可检测到无定形材料的大细胞内聚合物。 Burgdorferi抗体。 B.Burgdorferi IRS从Kupffer细胞的再介缓在接种细菌接种之前在RPMI培养基中孵育24小时，是阴性的。本研究中获得的结果表明，在没有血清因子的情况下，大鼠肝巨噬细胞有效地吸收和杀死博洛雷雷。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号