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首页> 外文期刊>Infection and immunity >Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.
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Intermedilysin, a novel cytotoxin specific for human cells secreted by Streptococcus intermedius UNS46 isolated from a human liver abscess.

机译：含有从人肝脓肿分离的链球菌UNS46分泌的人细胞特异的新型细胞毒素。

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摘要

A novel cytotoxin (intermedilysin) specific for human cells was identified as a cytolytic factor of Streptococcus intermedius UNS46 isolated from a human liver abscess. Intermedilysin caused human cell death with membrane blebs. Intermedilysin was purified from UNS46 culture medium by means of gel filtration and hydrophobic chromatography. The purified toxin was resolved into major and minor bands of 54 and 53 kDa, respectively, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These proteins reacted with an antibody against intermedilysin. Five internal peptide fragments of intermedilysin were sequenced and found to have 42 to 71% homology with the thiol-activated cytotoxin pneumolysin. However, the action of intermedilysin differed from that of thiol-activated cytotoxins, especially in terms of a lack of activation by dithiothreitol and resistance to treatments with N-ethylmaleimide and 5,5'-dithio-bis-(2-nitrobenzoic acid), although cholesterol inhibited the toxin activity. Intermedilysin was potently hemolytic on human erythrocytes but was 100-fold less effective on chimpanzee and cynomolgus monkey erythrocytes. Intermedilysin was not hemolytic in nine other animal species tested. Since human erythrocytes treated with trypsin were far less sensitive to intermedilysin than were the intact cells, a cell membrane protein(s) may participate in the intermedilysin action. These data demonstrated that intermedilysin is distinguishable from all known bacterial cytolysins.
机译：对人细胞特异的一种新型细胞毒素（含Imperallysin）被鉴定为中间体Unt46中中期Unt46的细胞分解因子，从人肝脓肿中分离。含有膜脑膜的人细胞死亡引起了人类细胞死亡。通过凝胶过滤和疏水色谱法从Unt46培养基中纯化含量素。将纯化的毒素分别通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分别分别由十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分解为54和53kDa的主要条带。这些蛋白质与抗体的抗体反应。测序中含量的五个内部肽片段，发现与硫醇活化细胞毒素肺素具有42至71％的同源性。然而，中间胞苷的作用与硫醇活化细胞毒素的作用不同，特别是在缺乏通过二噻唑醇和耐受N-乙基马来酰亚胺的处理和5,5'-二苯甲酸（2-硝基苯甲酸）的抗性而导致的。虽然胆固醇抑制了毒素活动。含有人红细胞的纯粹溶血性溶血，但在黑猩猩和Cynomolgus monkey红细胞上的效果较小。含有九种检测的其他动物物种的含溶血性。由于用胰蛋白酶处理的人红细胞对中于单独素敏感而不是完整的细胞，因此细胞膜蛋白可以参与内含物素作用。这些数据表明，中间素与所有已知的细菌胞嘧啶可区分。

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