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首页> 外文期刊>Infection and immunity >Analysis of proteins encoded by the ptx and ptl genes of Bordetella bronchiseptica and Bordetella parapertussis.
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Analysis of proteins encoded by the ptx and ptl genes of Bordetella bronchiseptica and Bordetella parapertussis.

机译：Bordetella Bronchiseptica和Bordetella Parapertussis的PTX和PTL基因编码的蛋白质分析。

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Bordetella pertussis is the only bacteria] species which is known to produce pertussis toxin (PT); however, both Bordetella bronchiseptica and Bordetella parapertussis contain regions homologous to the ptx genes of B. pertussis that encode the toxin subunits. After finding that several children with B. parapertussis infections exhibited modest antibody titers to PT, we examined the ptx genes of both B. parapertussis and B. bronchiseptica to determine whether they would encode stable, functional proteins even though their promoters are thought to be inactive under the conditions that have been examined. We inserted a functional promoter directly upstream of the ptx-ptl region of both species and examined culture supernatants of the resulting strains for PT activity. Biologically active PT was found in the culture supernatants of both engineered species. The toxin encoded by the B. parapertussis ptx genes appeared more labile in culture supernatants than did toxin produced by either B. pertussis or the engineered strain of B. bronchiseptica. This lability might be due to the lack of a full-length S2 subunit. We also investigated the ptl genes of these species, which are necessary for the secretion of this toxin, and found that both B. bronchiseptica and B. parapertussis contain at least certain of these genes, including ptlE and ptlF. Moreover, B. bronchiseptica appeared to contain all essential ptl genes since the introduction of a functional promoter directly upstream of the ptx-ptl region resulted in both production and efficient secretion of toxin. These results indicate that despite a number of amino acid changes in the sequences of the toxins, the toxins encoded by B. bronchiseptica and B. parapertussis are active.
机译：Bordetella pertussis是唯一已知的植物毒素（PT）的物种;然而，Bordetella Bronchiseptica和Bordetella Parapertussis都包含与编码毒素亚基的B.Pertussis的PTX基因同源的区域。在发现具有B. parapertussis感染的几个孩子呈现适度的抗体滴度后，我们检查了B.Parapertussis和B.Bronchiseptica的PTX基因，以确定它们是否会编码稳定的功能蛋白，即使他们的启动子被认为是不活跃的在已经检查的条件下。我们在两种物种PTX-PTL区域的直接上游插入了官能启动子，并检查了Pt活性所得菌株的培养基。在两个工程化物种的培养上清液中发现了生物活性Pt。 B.Parapertassis PTX基因编码的毒素在培养上清液中出现比由B.Pertussis或B.Bronchiseptica的工程化菌株产生的毒素更加不稳定。这种可爱可能是由于缺乏全长S2亚基。我们还研究了这些物种的PTL基因，这对于该毒素的分泌是必要的，发现B.Bronchiseptica和B.Parapertussis含有至少某些这些基因，包括PTL和PTLF。此外，B.Bronchiseptica似乎含有所有必需的PTL基因，因为在PTX-PTL区域的直接上游引入官能启动子导致毒素的产生和有效分泌。这些结果表明，尽管毒素的序列中存在许多氨基酸变化，但B.Bronchiseptica和B.Parapertassis编码的毒素是活性的。

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