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首页> 外文期刊>Infection and immunity >Nucleotide sequence homology to pertussis toxin gene in Bordetella bronchiseptica and Bordetella parapertussis.
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Nucleotide sequence homology to pertussis toxin gene in Bordetella bronchiseptica and Bordetella parapertussis.

机译：支气管败血性博德特氏菌和副百日咳博德特氏菌中百日咳毒素基因的核苷酸序列同源性。

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Multiple strains of Bordetella parapertussis and B. bronchiseptica were examined for the presence of nucleotide sequences which hybridized with a cloned 4.5-kilobase (kb) fragment of B. pertussis DNA containing the genes responsible for pertussis toxin expression. All six B. parapertussis strains tested had nucleic acid sequences that hybridized with the cloned 4.5-kb fragment in Southern blot analyses. When the B. parapertussis DNA was digested with restriction endonuclease PstI, the pattern of hybridization was identical to that obtained with B. pertussis. Only five of the seven B. bronchiseptica strains tested had sequences that hybridized with the 4.5-kb fragment. Three of these B. bronchiseptica strains had a hybridization pattern identical to B. pertussis upon PstI digestion and Southern blot analyses. Two B. bronchiseptica strains were shown to lack a PstI cleavage site downstream from the region analogous to that coding for the pertussis toxin structural genes. Monoclonal antibody analyses were unable to detect pertussis toxin subunits S1 and S2 in Western blots with cellular material or culture supernatant from several B. bronchiseptica and B. parapertussis strains that possessed the DNA homologies. In addition, preliminary Northern hybridizations with RNA isolated from B. bronchiseptica and B. parapertussis strains suggested that the homologous regions were not transcribed. The data show that the gene coding for the toxic component of B. pertussis is common in other Bordetella species, though the gene probably is not expressed.
机译：检查了多株副百日咳博德特氏菌和支气管败血性博德特氏菌菌株的核苷酸序列的存在，该核苷酸序列与克隆的百日咳博德特氏菌DNA的4.5-千碱基（kb）片段杂交，该片段含有负责百日咳毒素表达的基因。在Southern印迹分析中，测试的所有六种副百日咳博德特氏菌菌株均具有与克隆的4.5-kb片段杂交的核酸序列。当用限制性核酸内切酶PstI消化副百日咳博德特氏菌DNA时，杂交模式与百日咳博德特氏菌获得的杂交模式相同。测试的七种支气管败血性博德特氏菌菌株中只有五种具有与4.5 kb片段杂交的序列。在PstI消化和Southern印迹分析中，这些支气管败血性博德特氏菌菌株中的三个具有与百日咳博德特氏菌相同的杂交模式。已显示出两个支气管败血性芽孢杆菌菌株在类似于编码百日咳毒素结构基因的区域下游缺乏PstI切割位点。单克隆抗体分析无法通过Western印迹法从含有几种具有DNA同源性的支气管败血性博德特氏菌和副百日咳博德特氏菌菌株的细胞材料或培养上清液中检测百日咳毒素亚基S1和S2。另外，与从支气管败血性博德特氏菌和副百日咳博德特氏菌菌株分离的RNA的初步Northern杂交表明，同源区没有被转录。数据表明，编码百日咳博德特氏菌毒性成分的基因在其他博德特氏菌中很常见，尽管该基因可能未表达。

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