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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of culture and the Gen-Probe PACE 2 assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis in endocervical specimens transported to a state health laboratory.
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Evaluation of culture and the Gen-Probe PACE 2 assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis in endocervical specimens transported to a state health laboratory.

机译：培养评价及探针调节2分析检测内部局部炎症标本中的Neisseria Gonorrhoeae和Chlamydia Throcomatis运输到国家健康实验室。

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The Gen-Probe PACE 2 assay (Gen-Probe Inc., San Diego, Calif.) was compared with culture for the detection of Neisseria gonorrhoeae in endocervical specimens that were mailed to the laboratory. During mail transport, the specimens were exposed to extremes of hot and cold weather for several days before arriving in the laboratory. Specimens on culture plates deteriorated during transport, as evidenced by many dead gonococcus-like colonies. The manufacturer's recommendation for reporting PACE 2 assay-positive results was modified to create a suspicious category for samples with relative light units near the positive cutoff value. Of a total of 4,869 specimens tested, 30 were positive by both methods and 102 were positive only by the PACE 2 assay. These additional 102 positive specimens were likely to be true positives, as indicated by several lines of indirect evidence, including detailed probe competition analysis, patient history, and the lack of false-positive results in hand-delivered specimens. Although Gen-Probe Inc. indicates that specimens are stable for up to 7 days, N. gonorrhoeae was easily detectable by the PACE 2 assay after 1 month of incubation at room temperature in the PACE 2 transport buffer. We also compared the Gen-Probe PACE 2 assay for Chlamydia trachomatis with culture on endocervical specimens delivered by same-day courier. Of 398 endocervical specimens tested, the PACE 2 assay detected 19 of 20 culture-positive samples. Although the assay failed to detect one culture-positive sample, it was able to detect two very weak culture-suspicious samples. Finally, PACE 2 assays for N. gonorrhoeae and C. trachomatis performed on the same samples indicated that the coinfection rate was 40% for women attending five family planning clinics. We concluded that the Gen-Probe PACE 2 assay system should be considered for use in testing those specimens that are transported to the laboratory through the mail.
机译：Gen-探测器PACE 2测定（Gen-Probe Inc.，Calif。在邮件运输过程中，在抵达实验室之前，标本暴露于极端的热天气和寒冷的天气。培养板上的标本在运输过程中恶化，如许多死亡淋巴结壁样殖民地所证明。制造商对报告速度2测定结果的建议被修改以创建一个可疑类别，用于具有附近正截止值的相对光单元。在测试的4,869种样本中，通过两种方法，30个是阳性的，只有速度2测定为阳性。这些额外的102个阳性标本可能是真正的阳性，如几种间接证据所示，包括详细的探针竞争分析，患者历史，以及在手工递送的标本中缺乏假冒阳性结果。虽然Gen-Probe Inc.表明样品稳定为最多7天，在步骤2输送缓冲液中室温下孵育1个月后，速度2测定易于检测到N.淋病。我们还将Gen-Probe Pace 2测定与由当天快递员提供的内部截止物标本的培养物进行衣原体2分析。在测试的398个内截检标本中，PACE 2测定检测到20个培养阳性样品中的19个。虽然测定未能检测到一种培养阳性样品，但它能够检测两个非常弱的培养物样品。最后，在同一样品中进行的N.淋病和C.Gonorrhoeae和C.的测定结果表明，参加五个计划生育诊所的妇女的繁殖率为40％。我们得出结论，Gen-Probe Pace 2测定系统应考虑用于测试通过邮件运送到实验室的样本。

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