Purification of listeriolysin O and development of an immunoassay for diagnosis of listeric infections in sheep.

J C Low; R C Davies; W Donachie

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Purification of listeriolysin O and development of an immunoassay for diagnosis of listeric infections in sheep.

机译：Listeriolysin o的纯化和免疫测定的开发，以诊断绵羊中耳咽部感染。

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A protein of 58,000-Da molecular mass was purified from the supernatant fluid of a dialysis sac culture of Listeria monocytogenes by cation-exchange chromatography. The purified protein, homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and possessing the characteristics of listeriolysin O (LLO), was used to develop an indirect enzyme-linked immunosorbent assay. Anti-LLO antibodies were shown to be consistently produced in sheep after experimental challenge with L. monocytogenes serovar 4b. The assay also successfully detected and measured specific anti-LLO antibodies in the sera of silage-fed sheep among which listeric enteritis and abortions had occurred.

机译：通过阳离子交换色谱法从李斯特菌单核细胞增生的透析囊培养的上清液中纯化58,000 -DA分子量的蛋白质。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳和具有Listeriolysin O（LLO）特征的纯化蛋白，用于开发间接酶联免疫吸附测定。显示抗LLO抗体在用L.单核细胞增生塞洛伐克4b的实验攻击之后持续生产绵羊。在青贮饲养绵羊血清中也成功地检测和测量了特异性的抗LLO抗体，其中发生了肠炎和堕胎。

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《Journal of Clinical Microbiology》 |1992年第10期|共4页
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J C Low; R C Davies; W Donachie;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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6. Purification of listeriolysin O and development of an immunoassay for diagnosis of listeric infections in sheep. [O] . J C Low, R C Davies, W Donachie 1992

机译：李斯特菌溶血素O的纯化和免疫测定方法的开发用于诊断绵羊李斯特菌感染。
7. Purification of listeriolysin O and development of an immunoassay for diagnosis of listeric infections in sheep [O] . J C Low, R C Davies, W Donachie 1992

机译：纯化Listeriolysin o的纯化和免疫测定的开发，以诊断绵羊中耳听力的诊断

Purification of listeriolysin O and development of an immunoassay for diagnosis of listeric infections in sheep.

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