...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Factors influencing the infectivity of Chlamydia pneumoniae elementary bodies on HL cells.
【24h】

Factors influencing the infectivity of Chlamydia pneumoniae elementary bodies on HL cells.

机译：影响衣原体肺炎肺炎脑血管型血管基团对HL细胞感染的因素。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The influence of variations in the pH, NaCl concentration, temperature, and concentrations of calcium and magnesium ions on the survival of Chlamydia pneumoniae elementary bodies (EBs) outside the host cells was investigated. The survival was determined after various incubation periods by counting the inclusion-forming units after C. pneumoniae was cultured for 72 h on monolayers of HL cells. The normal physiological conditions were restored prior to infecting the HL cells with C. pneumoniae. Declines in the infectivities of C. pneumoniae EBs were observed at pH values of lower than 5 and higher than 8 or at NaCl concentrations of less than 80 mM. The viability of C. pneumoniae EBs in SPG medium decreased as the temperature and/or incubation period increased. Incubation temperatures of up to 20 degrees C and incubation periods of up to 48 h did not affect the viability of C. pneumoniae. One hundred percent of the C. pneumoniae EBs were infective after 1 h of incubation at 35 degrees C, whereas 90, 50, and 40% survived after incubations of 8, 24, and 48 h, respectively. The viability of C. pneumoniae was unaffected within the investigated range of Ca2+ and Mg2+ ion concentrations in the medium. The presence of 10% fetal calf serum in the incubation medium had a stabilizing effect on the viability of C. pneumoniae. This effect became more pronounced as the incubation period increased.
机译：研究了钙，NaCl浓度，温度和浓度在宿主细胞外部钙和镁离子浓度对冬式细胞外部体内（EBS）存活的影响。通过在将含有成形单位进行培养72小时后，通过计数填充形成单元在单层培养72小时后，在各种孵育期后测定存活。用C.肺炎感染HL细胞之前恢复正常的生理条件。在低于5的pH值下观察到C.肺炎肺炎EBS的感染率下降，高于8或小于80mm的NaCl浓度。随着温度和/或培养期增加，SPG培养基中的C.肺炎肺炎的活力降低。温育温度高达20℃和高达48小时的孵育期不影响C.肺炎的活力。在35℃温育1小时后，百分之百C.肺炎肺炎EBS感染，而90,50％和40％，分别在8,24和48小时后存活。 C.肺炎的可行性在培养基中的Ca2 +和Mg2 +离子浓度的研究范围内不受影响。在孵育培养基中存在10％胎牛血清的存在对C.肺炎的可行性稳定作用。随着潜伏期的增加，这种效果变得更加明显。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号