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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of a competitive enzyme immunoassay for detection of Coxiella burnetii antibody in animal sera.
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Evaluation of a competitive enzyme immunoassay for detection of Coxiella burnetii antibody in animal sera.

机译：竞争酶免疫测定检测动物血清中Coxiella Burnetii抗体的竞争性酶免疫测定。

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A competitive enzyme immunoassay (CEIA) was established and compared with other serological techniques for detecting Coxiella burnetii antibody in camels, goats, and sheep. This technique was evaluated because a conjugated anti-camel immunoglobulin was not available to serve as a direct signal for the demonstration of antigen-antibody reaction. A C. burnetii antibody-positive human serum and a peroxidase-conjugated anti-human immunoglobulin G were used as an indicator system competing against antibody in animal serum or as an indicator of the absence of antibody. Sera were considered antibody positive when the A414 of the test sera plus the competing positive antibody was less than or equal to 50% of the A414 of the negative-control serum plus the competing antibody. Antibody to C. burnetii was repeatedly demonstrated in 66% of camel serum samples (n = 200) by the CEIA. Among 48 camel serum samples, 71% were positive for antibody by CEIA versus 65% by EIA using peroxidase-labeled protein A. The CEIA detected C. burnetii antibody in 63% of sheep serum samples (n = 40) and in 50% of goat serum samples (n = 96), while the indirect fluorescent-antibody technique detected antibody in 38% of sheep and 34% of goat serum samples and the EIA detected antibody in 50% of sheep and 35% of goat serum samples. These data indicate that the CEIA is a reliable and sensitive technique for demonstrating C. burnetii antibody in camels, sheep, and goats.
机译：建立了一种竞争性酶免疫测定（CEIA），并与其他血清学技术进行了比较，用于检测骆驼，山羊和绵羊的Coxiella Burnetii抗体。评估该技术，因为不可用缀合的抗骆驼免疫球蛋白作为用于证明抗原 - 抗体反应的直接信号。将C.Burnetii抗体阳性人血清和过氧化物酶 - 缀合的抗人免疫球蛋白G作为竞争于动物血清中抗体的指示系统，或作为没有抗体的指示剂。当试验血清的A414竞争阳性抗体的A414小于或等于负对照血清A414的50％时，血清被认为是抗体阳性。 C.伯爵抗体在Ceia的66％的骆驼血清样品（n = 200）中赘桃蛋白抗体。在48个骆驼血清样品中，使用过氧化物酶标记的蛋白A，CEIA的CEIA与65％的抗体阳性为阳性。在63％的绵羊血清样品（n = 40）中检测到C. Burnetii抗体和50％山羊血清样品（n = 96），而间接荧光抗体技术在38％的绵羊中检测到抗体，34％的山羊血清样品和EIa检测到50％的绵羊和35％的山羊血清样品中的抗体。这些数据表明，CEIA是在骆驼，绵羊和山羊中展示C.Burnetii抗体的可靠和敏感的技术。

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