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首页> 外文期刊>Journal of bacteriology >Masking of Bacillus megaterium KM Membrane Reduced Nicotinamide Adenine Dinucleotide Oxidase and Solubilization Studies
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Masking of Bacillus megaterium KM Membrane Reduced Nicotinamide Adenine Dinucleotide Oxidase and Solubilization Studies

机译：髓杆菌米膜的掩蔽减少烟酰胺腺嘌呤二核苷酸氧化酶和溶解研究

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摘要

Solubilization of a reduced nicotinamide adenine dinucleotide (NADH)-2,6 dichlorophenol indophenol (DCIP) oxidoreductase associated with the membrane NADH oxidase system of Bacillus megaterium KM was effected by treatment with 0.2% sodium deoxycholate, 8 m urea, or buffer (pH 9.0) in the presence of ethyl-enediaminetetraacetate. These treatments inactivated membrane NADH oxidase. It was found that membrane NADH oxidase and NADH-DCIP oxidoreductase were masked in membranes. Several procedures, including brief sonic oscillation, treatment with 0.05% deoxycholate, prolonged stirring at 4 C with 10% glycerol, and washing in the absence of Mg2+, unmasked the oxidase and oxidoreductase activities. It was necessary to study the masking and unmasking of these activities to quantitate adequately the effects of solubilization procedures. Further information on the localization of oxidase and oxidoreductase in subcellular fractions and the effects of electron transport inhibitors on NADH oxidation was also obtained.
机译：通过用0.2％脱氧胆酸钠治疗，通过用0.2％脱氧胆酸钠，8米进行溶解与芽孢杆菌牛氧化酶 km的膜NADH氧化酶系统相关的减少的烟酰胺腺嘌呤二核苷酸（NADH）-2,6二氯苯酚靛蓝醇（DCIP）氧化还原酶尿素或缓冲液（ P H 9.0）在存在乙基 - 己二胺四乙酸盐存在下。这些处理灭活膜NADH氧化酶。发现在膜中掩盖膜NADH氧化酶和NADH-DCIP氧化酶。几种程序，包括简短的声音振荡，用0.05％脱氧胆酸盐处理，在4℃下延长搅拌10％甘油，并在不存在Mg 2 + ，揭开氧化酶和氧化酶和氧化酶活性。有必要研究这些活动的掩蔽和揭露，以定量溶解程序的影响。还获得了有关亚细胞分数中氧化酶和氧化酶酶的局部化的进一步信息以及电子传输抑制剂对NADH氧化的影响。

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