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首页> 外文期刊>Journal of bacteriology >Streptomycin-Suppressible Lethal Mutations in Escherichia coli
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Streptomycin-Suppressible Lethal Mutations in Escherichia coli

机译：大肠杆菌中的链霉素抑制致命突变

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Forty-one mutants have been isolated which require streptomycin for growth on complete medium. These streptomycin-suppressible lethal mutations are located randomly around the Escherichia coli genetic map; during growth in liquid culture, they exhibit a variety of responses to the removal of streptomycin as judged by turbidity, cell morphology, and macromolecular synthesis. In particular, some mutants are primarily affected in protein or ribonucleic acid (RNA) synthesis (or both), one in deoxyribonucleic acid synthesis, and two in lipid synthesis. Ten mutants affected in protein synthesis were examined for the activities of all twenty aminoacyl-transfer RNA synthetases, and three were found to have altered glutamyl-transfer RNA synthetase activities. The advantages of this method for isolating a wide variety of conditional lethal mutants are discussed.
机译：已经分离了四十一体突变体，其需要在完全培养基上进行链霉素进行生长。这些链霉素抑制的致命致命突变在大肠杆菌/ em>遗传图谱周围随机定位;在液体培养的生长期间，它们表现出各种反应，以通过浊度，细胞形态学和大分子合成判断而去除链霉素。特别地，一些突变体主要在蛋白质或核糖核酸（RNA）合成（或两者）中受到脱氧核糖核酸合成的影响，以及两种在脂质合成中。检查在蛋白质合成中影响的十个突变体用于所有20个氨基酰基转移RNA合成酶的活动，并发现三种具有改变的谷氨酸转移RNA合成酶活性。讨论了该方法用于隔离各种条件致死突变体的优点。

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