• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Identification of suppressive components in 'Haemophilus somnus' fractions which inhibit bovine polymorphonuclear leukocyte function.
【24h】

Identification of suppressive components in 'Haemophilus somnus' fractions which inhibit bovine polymorphonuclear leukocyte function.

机译：抑制抑制牛多核白细胞功能的抑制成分抑制成分。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

"Haemophilus somnus" fractions which inhibited iodination of protein by bovine polymorphonuclear leukocytes were isolated by heat extracting a washed bacterial suspension at 60 degrees C or incubating the bacterial suspension at 37 degrees C and were partially purified by ultrafiltration. The components in each fraction were separated by reverse-phase high-performance liquid chromatography and identified as ribonucleotides, a ribonucleoside, and purine and pyrimidine bases. Most of the compounds were found to be inhibitory to iodination in a dose-dependent manner. When the effect of each component on iodination at the concentrations present in "H. somnus" fractions was determined, it was found that guanine and GMP were the components responsible for most of the suppression in the fraction isolated by heat extraction, whereas guanine and adenine were the major inhibitory components in the fraction isolated by incubation at 37 degrees C.
机译：通过将洗涤的细菌悬浮液在60℃下萃取洗涤的细菌悬浮液或在37℃下将洗涤的细菌悬浮液加热或在37℃下孵育细菌悬浮液，并通过超滤部分纯化，抑制蛋白质的碘化蛋白质抑制蛋白质的蛋白质的馏分。通过反相高效液相色谱法分离各部分中的组分，并鉴定为核糖核苷酸，核糖核苷和嘌呤和嘧啶碱基。发现大多数化合物以剂量依赖性方式抑制碘化。当确定每种组分对“H.SOMNUS”级分的浓度下碘化的影响时，发现鸟嘌呤和GMP是由热萃取分离的馏分中的大部分抑制的组分，而鸟嘌呤和腺嘌呤是在37℃的孵育中分离的级分中的主要抑制成分。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号