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首页> 外文期刊>Applied and Environmental Microbiology >Existence of a new type of sulfite oxidase which utilizes ferric ions as an electron acceptor in Thiobacillus ferrooxidans.
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Existence of a new type of sulfite oxidase which utilizes ferric ions as an electron acceptor in Thiobacillus ferrooxidans.

机译：一种新型亚硫酸盐氧化酶的存在，其利用铁离子作为硫代毒素铁氧播尼的电子受体。

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摘要

A new type of sulfite oxidase which utilizes ferric ion (Fe3+) as an electron acceptor was found in iron-grown Thiobacillus ferrooxidans. It was localized in the plasma membrane of the bacterium and had a pH optimum at 6.0. Under aerobic conditions, 1 mol of sulfite was oxidized by the enzyme to produce 1 mol of sulfate. Under anaerobic conditions in the presence of Fe3+, sulfite was oxidized by the enzyme as rapidly as it was under aerobic conditions. In the presence of o-phenanthroline or a chelator for Fe2+, the production of Fe2+ was observed during sulfite oxidation by this enzyme under not only anaerobic conditions but also aerobic conditions. No Fe2+ production was observed in the absence of o-phenanthroline, suggesting that the Fe2+ produced was rapidly reoxidized by molecular oxygen. Neither cytochrome c nor ferricyanide, both of which are electron acceptors for other sulfite oxidases, served as an electron acceptor for the sulfite oxidase of T. ferrooxidans. The enzyme was strongly inhibited by chelating agents for Fe3+. The physiological role of sulfite oxidase in sulfur oxidation of T. ferrooxidans is discussed.
机译：一种新型的亚硫酸盐氧化酶，其利用铁离子（Fe3 +）作为电子受体被发现在铁生长的硫昔洛呋喃氧化钒中。它在细菌的血浆膜中局部化，并且在6.0时具有pH值。在有氧条件下，通过酶氧化1摩尔亚硫酸盐以产生1摩尔硫酸盐。在Fe3 +存在下的厌氧条件下，亚硝酸盐通过酶氧化，因为它在有氧条件下。在O-菲咯啉的存在或Fe2 +的螯合剂存在下，通过该酶在亚硝酸盐氧化期间观察到Fe2 +的生产不仅是厌氧条件，而且在厌氧条件下进行了不良条件。在没有O-菲咯啉的情况下没有观察到Fe2 +生产，表明通过分子氧快速再氧化Fe 2 +。既不是细胞色素C也不是铁氰化物，两者都是用于其他亚硫酸盐氧化酶的电子受体，用作T.Frooxidans的亚硫酸盐氧化酶的电子受体。通过螯合剂螯合Fe3 +强烈抑制酶。讨论了亚硫酸盐氧化酶在硫氧化物硫氧化中的生理作用。

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