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首页> 外文期刊>Journal of Clinical Microbiology >Growth and Amino Acid Requirements of Various Strains of Group B Streptococci
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Growth and Amino Acid Requirements of Various Strains of Group B Streptococci

机译：B组群中各种菌株的生长和氨基酸要求

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A chemically defined medium (FMC; B. Terleckyj, N. P. Willett, and G. D. Shockman, Infect. Immun. >11:649-655, 1975) was used to compare the growth and amino acid requirements of 16 strains of group B streptococci, consisting of both laboratory-passaged organisms and fresh clinical isolates from adult and neonatal infections. The 5 standard Lancefield immunizing strains of group B streptococci, 090 (Ia), H36B (Ib), A909 (Ic), 18RS21 (II), and D136C (III), had doubling times in FMC (28 to 36 min) similar to those observed in Todd-Hewitt glucose broth (24 to 30 min). Similar doubling times were obtained with 11 clinical isolates growing in Todd-Hewitt glucose broth and FMC. The optimum buffering capacity of FMC was provided by 0.06 M sodium phosphate, and 1% glucose gave maximum cell yield. The group B streptococci, with minor exceptions, were very homogeneous in their amino acid requirements under both aerobic and anaerobic growth conditions. Phenylalanine, tyrosine, tryptophan, glutamate, arginine, valine, leucine, lysine, methionine, isoleucine, cystine, and histidine were required by all 16 strains under both aerobic and anaerobic growth conditions. In addition, threonine was required by all strains under aerobic growth conditions, whereas only 9 strains required threonine under anaerobic conditions. Serine was required by only 3 type III fresh clinical isolates aerobically, but not anaerobically. A requirement for glycine varied from strain to strain, apparently influenced by the oxidation-reduction potential of the growth medium.
机译：化学定义的培养基（FMC; B. Terleckyj，NP Willett和Gd Shockman，感染。Immun。> 11 ：649-655,1975）用于比较16株的生长和氨基酸要求B组链球菌组成，由实验室传代的生物和来自成人和新生儿感染的新鲜临​​床分离株组成。 B组链球菌，090（IA），H36B（IB），A909（IC），18RS21（II）和D136C（III）的5标准兰氏植物免疫菌株在FMC（28至36分钟）中具有倍增时间（28至36分钟）在Todd-Hewitt葡萄糖肉汤（24至30分钟）中观察到的那些。在Todd-Hewitt葡萄糖肉汤和FMC中生长的11个临床分离物，获得了类似的倍增时间。 FMC的最佳缓冲能力由0.06M磷酸钠提供，1％葡萄糖产生最大的电池产率。 B组链球菌，含小例外，在有氧和厌氧生长条件下，在它们的氨基酸要求中非常均匀。在有氧和厌氧生长条件下，所有16株都需要苯丙氨酸，酪氨酸，缬氨酸，谷氨酸，精氨酸，缬氨酸，亮氨酸，赖氨酸，甲硫氨酸，异亮氨酸，胱氨酸和组氨酸。此外，所有菌株都需要在有氧生长条件下需要苏氨酸，而仅在厌氧条件下仅需要9个菌株。只有3型III型新鲜临床分离株需要丝氨酸，但不是厌氧。甘氨酸的要求因菌株而变化，显然受生长培养基的氧化还原电位的影响。

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