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首页> 外文期刊>Infection and immunity >Amino acid requirements of Streptococcus mutans and other oral streptococci.
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Amino acid requirements of Streptococcus mutans and other oral streptococci.

机译：变形链球菌和其他口服链球菌的氨基酸需求。

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The amino acid requirements of Streptococcus mutans strains AHT, OMZ-61, FA-1, BHT, GS-5, JC-2, Ingbritt, At6T, OMZ-176, 6715, Streptococcus salivarius HHT, Streptococcus sanguis OMZ-9, and strain 72x46 were determined in a chemically defined medium. When grown anaerobically in the presence of sodium carbonate (or bicarbonate for a few strains), few amino acids were required. All strains tested required cystine (or cystine) as a nutrient. Three strains (S. mutans OMZ-176, FA-1, and BHT) required glutamate (and/or glutamine). A third amino acid (lysine for S. mutans FA-1 and histidine for S. mutans OMZ-176) was required by two of the three strains which required glutamate. The amino acids mentioned above were required for all conditions of incubation (and inoculum) tested. The requirements for several other amino acids were conditional, that is, dependent on the incubation conditions and inoculum used. For example, when carbonate was not added, glutamate was required by S. mutans GS-5. Aerobic incubations, with carbonate or bicarbonate added, resluted in requirements for glutamate and leucine by several strains. With these incubation conditions, one strain required isoleucine (S. mutans FA-1), another valine (S. mutans AHT), and a third tyrosine (72x46). Aerobic incubations in the absence of carbonate or bicarbonate further increased the number of amino acids required by several strains. Furthermore, when stationary-phase cultures replaced exponentially growing cultures as an inoculum, several strains required additional amino acids, presumably for the initiation of growth.
机译：变形链球菌菌株AHT，OMZ-61，FA-1，BHT，GS-5，JC-2，Ingbritt，At6T，OMZ-176、6715，唾液链球菌HHT，血链球菌OMZ-9和菌株的氨基酸需求在化学成分确定的培养基中确定72x46。当在碳酸钠（或一些菌株的碳酸氢盐）存在下厌氧生长时，所需氨基酸很少。所有测试的菌株都需要胱氨酸（或胱氨酸）作为营养物质。三种菌株（变形链球菌OMZ-176，FA-1和BHT）需要谷氨酸盐（和/或谷氨酰胺）。需要谷氨酸的三个菌株中的两个需要第三个氨基酸（变形链球菌FA-1为赖氨酸，变形链球菌OMZ-176为组氨酸）。上述氨基酸是测试的所有孵育条件（和接种物）所必需的。其他几种氨基酸的要求是有条件的，即取决于孵育条件和所用接种物。例如，当不添加碳酸盐时，变形链球菌GS-5需要谷氨酸盐。添加了碳酸盐或碳酸氢盐的有氧培养被几种菌株重新稀释成对谷氨酸和亮氨酸的要求。在这些温育条件下，一种菌株需要异亮氨酸（变形链球菌FA-1），另一种缬氨酸（变形链球菌AHT）和第三种酪氨酸（72x46）。在没有碳酸盐或碳酸氢盐的情况下，需氧培养进一步增加了几种菌株所需的氨基酸数量。此外，当固定相培养物代替指数生长的培养物作为接种物时，一些菌株需要额外的氨基酸，大概是为了开始生长。

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