Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles.

R F DAmato; L A Eriquez; K M Tomfohrde; E Singerman

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Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles.

机译：用酶促谱快速鉴定Neisseria Gonorrhoeae和Neisseria Meningitidis。

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The enzymatic profiles of Neisseria gonorrhoeae, N. meningitidis, and related species were determined, using a total of 48 chromogenic substrates. Enzyme classes assayed for included glycosidases, aminopeptidases, phosphoamidases, proteases, lipases, esterases, and aryl sulfatase. A final test selection of 10 substrates, based upon their differential and reproducible characteristics, allowed the separation of N. gonorrhoeae and N. meningitidis from each other and from all species tested within 4 h after primary isolation on modified Thayer-Martin medium. The need for subculturing suspect colonies from modified Thayer-Martin medium to chocolate medium with a subsequent loss of 18 to 24 h of identification is eliminated.

机译：使用总共48个发色基质测定Neisseria淋病，N.脑膜炎和相关物种的酶促谱。用于包括糖苷酶，氨基肽，磷酰胺酶，蛋白酶，脂肪酶，酯酶和芳基磺酸糖酶的酶类。基于它们的差异和可重复的特性，允许彼此分离N.淋病和N.脑膜炎的最终试验选择10个底物。在修饰的Thayer-Martin培养基上初次分离后，在4小时内测试的所有物种。消除了从改性的Thayer-Martin培养基到巧克力培养基中培养的需要，随后损失为18至24小时的识别。

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《Journal of Clinical Microbiology》 |1978年第1期|共5页
作者
R F DAmato; L A Eriquez; K M Tomfohrde; E Singerman;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Identification of Regions of the Chromosome of Neisseria meningitidis and Neisseria gonorrhoeae Which Are Specific to the Pathogenic Neisseria Species [J] . Agnes Perrin, Xavier Nassif, Colin Tinsley Infection and immunity . 1999,第11期

机译：脑膜炎奈瑟氏球菌和淋病奈瑟氏球菌的染色体区域的鉴定，这些区域特定于致病性奈瑟氏菌属
2. Critical determinants of host receptor targeting by Neisseria meningitidis and Neisseria gonorrhoeae: identification of Opa adhesiotopes on the N-domain of CD66 molecules. [J] . Virji M, Evans D, Hadfield A, Molecular Microbiology . 1999,第3期

机译：脑膜炎奈瑟氏球菌和淋病奈瑟氏球菌靶向宿主受体的关键决定因素：CD66分子N结构域上的Opa粘附位的鉴定。
3. Identification of some basic extractable compounds produced by Neisseria gonorrhoeae and Neisseria meningitidis in a defined medium. [J] . J B Brooks, D S Kellogg, G Choudhary, Journal of Clinical Microbiology . 1978,第5期

机译：在确定的培养基中鉴定淋病奈瑟氏球菌和脑膜炎奈瑟氏球菌产生的一些基本可提取化合物。
4. Utility of Random Forest combined with Infusion Electrospray MS and MALDI-TOF MS for rapid Classification of Neisseria meningitidis strains [C] . Adrian R Woolfitt, Hercules Moura, Bryan A Parks, ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：随机森林的效用联合输注电喷雾MS和MALDI-TOF MS，用于快速分类Neisseria Meningitidis菌株
5. A rapid method for detecting single nucleotide polymorphisms using antimicrobial resistance in Neisseria gonorrhoeae as a model. [D] . Cullingham, Kyle J. G. 2005

机译：一种使用淋病奈瑟氏菌为模型的抗药性检测单核苷酸多态性的快速方法。
6. Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles. [O] . R F DAmato, L A Eriquez, K M Tomfohrde, 1978

机译：使用酶谱快速鉴定淋病奈瑟氏球菌和脑膜炎奈瑟氏球菌。
7. Critical determinants of host receptor targeting by Neisseria meningitidis and Neisseria gonorrhoeae : identification of Opa adhesiotopes on the N-domain of CD66 molecules [O] . Mumtaz Virji, Debbie Evans, Andrea Hadfield, 1999

机译：Neisseria Meningitidis和Neisseria淋病术治疗宿主受体的关键决定因素：鉴定CD66分子N-结构域的OPA杂皮飞机
8. Heterogeneity of Molecular Size and Antigenic Expression within Lipooligosaccharides of Individual Strains of Neisseria gonorrhoeae and Neisseria meningitidis. [R] . Schneider, H., Hale, T. L., Zollinger, W. D., 1984

机译：淋病奈瑟菌和脑膜炎奈瑟球菌个别低密度脂蛋白的分子大小和抗原表达的异质性。

Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles.

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