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首页> 外文期刊>Scientific reports. >MicroRNA in sperm from Duroc, Landrace and Yorkshire boars
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MicroRNA in sperm from Duroc, Landrace and Yorkshire boars

机译：微RNA从杜洛克，长白猪和约克夏公猪精子

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Sperm contain microRNAs (miRNAs), which may have roles in epigenetic control. Regarding phylogenetic relationships among various swine breeds, Yorkshire and Landrace, are considered phenotypically and genetically very similar, but distinctly different from Duroc. The objective of the present study was to compare abundance of boar sperm miRNAs in these three breeds. Overall, 252 prioritized miRNAs were investigated using real-time PCR; relative expression of miRNAs in sperm was similar in Yorkshire and Landrace boars, but significantly different compared to Duroc. Seventeen miRNAs (hsa-miR-196a-5p, hsa-miR-514a-3p, hsa-miR-938, hsa-miR-372-3p, hsa-miR-558, hsa-miR-579-3p, hsa-miR-595, hsa-miR-648, hsa-miR-524-3p, hsa-miR-512-3p, hsa-miR-429, hsa-miR-639, hsa-miR-551a, hsa-miR-624-5p, hsa-miR-585-3p, hsa-miR-508-3p and hsa-miR-626) were down-regulated (P??0.05; fold regulation ≤-2) in Yorkshire and Landrace sperm, compared to Duroc sperm. Furthermore, three miRNAs (hsa-miR-9-5p, hsa-miR-150-5p, and hsa-miR-99a-5p) were significantly up-regulated in Yorkshire and Landrace sperm compared to Duroc sperm, However, 240 miRNAs were not significantly different (within?+?2 fold) between Yorkshire and Landrace sperm. We concluded that miRNAs in sperm were not significantly different between Yorkshire and Landrace boars, but there were significant differences between those two breeds and Duroc boars. Furthermore, integrated target genes for selected down-regulated miRNAs (identified via an in-silico method) appeared to participate in spermatogenesis and sperm functions.
机译：精子含有microRNAs（miRNA），其可能在表观遗传控制中具有作用。关于各种猪品种，约克郡和地拉站的系统发育关系被认为是表型和遗传学上的，但与Duroc不同。本研究的目的是比较这三种品种中的丰富的公猪精子miRNA。总体而言，使用实时PCR研究了252个优先考虑的miRNA; MiRNA在精子中的相对表达在约克夏和Landrace公猪中类似，但与Duroc相比显着不同。 17米MIRNA（HSA-MIR-196A-5P，HSA-MIR-514A-3P，HSA-MIR-938，HSA-MIR-372-3P，HSA-MIR-558，HSA-MIR-579-3P，HSA-MIR -595，HSA-MIR-648，HSA-MIR-524-3P，HSA-MIR-512-3P，HSA-MIR-429，HSA-MIR-639，HSA-MIR-551A，HSA-MIR-624-5P ，与Duroc精子相比，HSA-MiR-508-3P和HSA-MIR-626）被下调（P？<？0.05;折叠调节≤-2），与Duroc精子相比，Handrace精子。此外，在约克郡和Landrace精子中，三种miRNA（HSA-MIR-9-5P，HSA-MIR-150-5P和HSA-MIR-99A-5P）与Duroc精子相比，Landrace精子有显着上调，然而，240 miRNA是约克郡和地兰精子之间没有显着差异（内部？+？2倍）。我们得出结论，约克郡和兰德斯公猪之间的精子中的miRNA没有显着差异，但这两种品种和杜隆公猪之间存在显着差异。此外，用于所选择的下调miRNA的集成靶基因（通过硅氧化物方法鉴定）似乎参与精子发生和精子功能。

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