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首页> 外文期刊>Balkan journal of medical genetics: BJMG >Relationship between chromosomal aberrations and gene expressions in the p53 pathway in chronic lymphocytic leukemia
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Relationship between chromosomal aberrations and gene expressions in the p53 pathway in chronic lymphocytic leukemia

机译：慢性淋巴细胞白血病P53途径中染色体畸变与基因表达的关系

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Chronic lymphocytic leukemia (CLL) is a neoplasm characterized by excessive accumulation of B lymphocytes in the peripheral blood, bone marrow and lymph nodes. We assessed the expressions of 22 genes in the p53 pathway in 30 CLL patients and 15 healthy subjects by a RT2 Profiler PCR (polymerase chain reaction) Array technique and their relation to cytogenetic aberrations detected by fluorescent in situ hybridization (FISH). Our Studenta??s t-test results indicated that ATM, ATR, BAX, CASP9, CDK4, CDKN2A, CHEK1, CHEK2, E2F3, MCL1, MDM2, MDM4, PCNA, RB1, P53 and BCL2 genes were statistically significant (p 0.001). For six genes (APAF1, CDKN1A, E2F1, GADD45A, PTEN and PTX3) were not statistically significant. The ATM, ATR, BAX, CASP9, CDK4, CDKN1A, CDKN2A, CHEK1, CHEK2, MDM2, MDM4, PCNA, RB1, P53, E2F1, GADD45A and BCL2 genes were found to be upregulated by the 2-á??á??Ct (relative fold change in gene expression) method. The highest up-regulation was detected in CDKN2A and BCL2 genes, 10.22- and 8.51-fold, respectively. On the other hand, the PTX3 gene with a fold regulation of 1.84 was found to the highest downregulation. Overall, the CDNK2A BCL2 and PTX3 genes are related to the mechanism of the disease in the p53 pathway and may be an important predictor of the prognosis of the disease. The BCL2 gene may be associated with increased risk of developing CLL. We suggest that the PTX3 gene may be considered as a marker associated with CLL disease. The CDKN2A gene expression seems to play a protective role in CLL.
机译：慢性淋巴细胞白血病（CLL）是一种肿瘤，其特征在于外周血，骨髓和淋巴结中的B淋巴细胞过度积累。我们通过RT2分析器PCR（聚合酶链反应）阵列技术评估了30个CLL患者和15名健康受试者的P53途径中的22种基因的表达及其与荧光原位杂交（鱼）检测到的细胞遗传学畸变的关系。我们的stuitenta ?? s t-test结果表明，ATM，ATR，BAX，CASP9，CDK4，CDKN2A，CHEK1，CHEK2，E2F3，MCL1，MDM2，MDM4，PCNA，RB1，P53和BCL2基因均有统计学意义（P <0.001 ）。对于六个基因（APAF1，CDKN1A，E2F1，GADD45A，PTEN和PTX3）没有统计学意义。发现ATM，ATR，BAX，CASP9，CDK4，CDKN1A，CDKN2A，CHEK1，CHEK2，MDM2，MDM4，PCNA，RB1，P53，E2F1，GADD45A和BCL2基因被2-áΔ?á?? CT（基因表达中的相对折叠变化）方法。在CDKN2A和BCL2基因中分别检测到最高上调，分别在10.22-和8.51倍。另一方面，发现具有1.84的折叠调节的PTX3基因在最高下调。总体而言，CDNK2A BCL2和PTX3基因与P53途径中疾病的机制有关，并且可能是该疾病预后的重要预测因子。 BCL2基因可能与发育CLL的风险增加有关。我们建议PTX3基因可以被认为是与CLL病相关的标志物。 CDKN2A基因表达似乎在CLL中发挥了保护作用。

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