• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Balkan journal of medical genetics: BJMG >The expression levels of microRNAs associated with T and B cell differentiation/stimulation in ankylosing spondylitis
【24h】

The expression levels of microRNAs associated with T and B cell differentiation/stimulation in ankylosing spondylitis

机译：与T和B细胞分化/刺激在强直性脊柱炎相关的微小RNA的表达水平

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Spondyloarthropathies (SpAs), are a group of chronic inflammatory diseases with a number of genetic, physiopathological, clinical and radiological features. Ankylosing spondylitis (AS) is the most common type of spondylo-arthropathies, and 90.0% of patients with ankylosing spondylitis are human leukocyte antigen-B27 (HLA-B2 7)-positive. In recent years, non-HLA genetic factors have been reported to have an effect on ankylosing spondylitis. MicroRNAs (miRNAs), are endogenous non coding RNA molecules containing 18-23 nucleotides that play a role in the post-transcriptional regulation of gene expression. In this study, we aimed to determine the expression levels of miRNAs associated with T- and B-cell differentiation/stimulation in peripheral blood mononuclear cells and their relationship with the etiology of the AS in patients and healthy controls. In a molecular study, peripheral blood mononuclear cell isolation, and total RNA isolation were performed first. In the second step, cDNA synthesis and quantitative real-time PCR (qPCR) expression analysis were completed. Ultimately, in the patient and control group, the expression levels of miR-142-5p and miR-143 were found to be significantly different (p 0.05). According to current knowledge, miR-142-5p andmiR-143 expressions were found to be important for those diseases that share similar etiology with AS. We suggest that miR-142-5p and miR-143 may play a role in the pathogenesis, especially miR- 142-5p may be a potential biomarker and a target molecule for the treatment.
机译：脊椎内疗法（SPAS）是一群慢性炎症疾病，具有许多遗传，物理病理学，临床和放射性特征。强直性脊柱炎（AS）是最常见的脊柱关节运动类型，> 90.0％的强直性脊柱炎患者是人白细胞抗原-B27（HLA-B2 7） - 阳性。近年来，据报道，非HLA遗传因素对强直性脊柱炎有影响。 microRNA（miRNA），是内源性非编码RNA分子，其含有18-23个核苷酸，其在基因表达的后转录调节中起作用。在这项研究中，我们旨在确定与外周血单核细胞中与T-和B细胞分化/刺激相关的miRNA的表达水平及其与患者和健康对照的病因的关系。在分子研究中，首先进行外周血单核细胞分离和总RNA分离。在第二步中，完成CDNA合成和定量实时PCR（QPCR）表达分析。最终，在患者和对照组中，发现miR-142-5p和miR-143的表达水平显着不同（p <0.05）。根据目前的知识，发现miR-142-5p和mir-143表达对那些与与此类似病程相似的疾病是重要的。我们建议miR-142-5p和miR-143可以在发病机制中发挥作用，特别是miR-142-5p可以是潜在的生物标志物和用于治疗的靶分子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号