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首页> 外文期刊>Biotechnology Reports >Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain
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Optimization of process parameters and fermentation strategy for xylanase production in a stirred tank reactor using a mutant Aspergillus nidulans strain

机译：使用突变体曲霉菌菌株搅拌釜反应器中木聚糖酶生产的工艺参数和发酵策略的优化

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The present work studied the optimization of aeration rate, agitation rate and oxygen transfer and the use of various batch fermentation strategies for xylanase production from a recombinant Aspergillus nidulans strain in a 3?L stirred tank reactor. Maximum xylanase production of 1250 U/mL with productivity of 313 U/mL/day was obtained under an aeration rate of 2 vvm and an agitation rate of 400?rpm using batch fermentation. The optimum volumetric oxygen transfer coefficient (ksubL/suba) for efficient xylanase production was found to be 38.6?hsup?1/sup. Fed batch mode and repeated batch fermentation was also performed with ksubL/suba was 38.6?hsup?1/sup. Xylanase enzyme productivity increased to 327 with fed batch fermentation and 373 U/mL/day with repeated batch fermentation. Also, maximum xylanase activity increased to 1410 U/mL with fed batch fermentation and 1572 U/mL with repeated batch fermentation.
机译：本工作研究了曝气率，搅拌率和氧气转移的优化，以及在3〜L搅拌釜反应器中的重组曲霉菌菌株中使用各种批量发酵策略的使用。在2VVM的曝气速率下获得1250u / ml的1250u / ml的最大木聚糖酶产生的1250 u / ml /天，使用分批发酵的搅拌速度为400μlΩ·rpm。有效的木聚糖酶产生的最佳体积氧转移系数（K L a）是38.6Ω·h α1。用K L A进行38.6Ω，还进行喂食批量模式和重复的分批发酵。用FED分批发酵增加到327的木聚糖酶生产率增加至373u / ml /天，重复的批量发酵。此外，最大木聚糖酶活性随着氟批批量发酵和1572u / ml增加至1410 u / ml，重复的分批发酵。

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