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首页> 外文期刊>Biotechnology & Biotechnological Equipment >Assessment of genetic diversity in Triticum urartu Thumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers
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Assessment of genetic diversity in Triticum urartu Thumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers

机译：利用起始密码子靶向多态性（SCOT）和CAAT-BOX衍生多态性（CBDP）标记，评估Triticum Urartu Thumanjan Ex Gandilyan进入的遗传多样性

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Among the wild relatives of wheat, Triticum urartu Tumanian ex Gandilyan is of interest to wheat breeders. Numerous works have focussed on its utilization for enrichment of the genetic variation of cultivated genotypes. In the present study, the genetic diversity and population structure in 85 accessions of T. urartu were investigated using start codon targeted (SCoT) polymorphism and CAAT-box derived polymorphism (CBDP) markers. Nineteen SCoT primers and fifteen CBDP primers amplified 185 and 141 polymorphic fragments with an average of 9.74 and 9.40 fragments per primer, respectively. The polymorphic information content ( PIC ) for the SCoT and CBDP primers ranged from 0.41 to 0.50 and 0.40 to 0.49, with the resolving power ( Rp ) ranging from 21.61 to 3.97 and 13.08 to 28.02, respectively. Neighbour-joining (NJ) based clustering grouped 72 accessions into two main clusters based on the two sets of markers and the combined data. Genetic relationships inferred from STRUCTURE analysis was matched with cluster analysis and principal coordinate analysis (PCoA), indicating that the accessions were grouped into two major clades and the grouping patterns were not correlated with the geographic origins. The results from this study revealed that Iranian T. urartu, especially Kerend-e-Gharb and Sisakht-Pataveh populations, can be interesting for wheat improvement. Hence, conservation of this region is recommended.
机译：在小麦的野生亲戚中，小麦育种者的小麦urartu tumanian ex甘狼是感兴趣的。许多作品侧重于其利用富集培养基因型的遗传变异。在本研究中，使用起始密码子靶向（SCOT）多态性和CAAT盒衍生的多态性（CBDP）标记来研究85例Ruartu遗传多样性和群体结构。十九个突出引物和十五个CBDP引物分别扩增185和141个多态性片段，平均每种引物平均为9.74和9.40片段。 SCOT和CBDP引物的多态信息含量（PIC）范围为0.41至0.50和0.40至0.49，分辨率分别为21.61至3.97和13.08至28.02至28.02。基于邻接（NJ）的聚类基于两组标记和组合数据分组为两个主要群集。从结构分析推断的遗传关系与集群分析和主坐标分析（PCOA）匹配，表明将进入分为两个主要的片状，分组模式与地理起源没有相关。本研究的结果透露，伊朗T. Urartu，特别是Kerend-E-Gharb和Sisakht-Pataveh种群，对小麦改善感兴趣。因此，建议保护该地区。

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