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首页> 外文期刊>BMC Infectious Diseases >Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China
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Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China

机译：中国黑龙江省海葵抗肠杆菌酸肠道复合物的金属-β-乳酰胺酶生产的分子特征

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BACKGROUND:Enterobacter cloacae complex (ECC) is one of the most common extended-spectrum β-lactamase and carbapenemase-producing pathogen that threatens millions of the elderly and vulnerable sick persons. The objective of this study was to perform the molecular characteristics of the carbapenem-resistant E. cloacae complex (CREC) emerged in Heilongjiang Province of China.METHODS:Six CREC strains were isolated from the patients with infectious diseases. The identities of ECC isolates were confirmed by sequencing the polymerase chain reaction (PCR) products of 16S rRNA gene. The characterization of the CREC isolates were analyzed by sequencing PCR products of the carbapenemase, ampC and fluoroquinolone resistance genes and performing multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) and whole genome sequencing.RESULTS:All 6 isolates harbored multiple resistance genes. Of them, 5 carried metallo-β-lactamases and one was blasubKPC-2/sub-positive. The levofloxacin and ciprofloxacin-resistant strains had substitutions of gyrA83, gyrA87, and parC80 in the quinolone-resistance determining regions. The MLST analyses revealed that 6 isolates belonged to five sequence types (ST520, ST528, ST1119, ST1120, and ST93) while the PFGE patterns of the isolates fallen into four clusters. The strain ST1120 was found to carry two separated plasmids that encode blasubNDM-1/sub and blasubIMP-4/sub.CONCLUSIONS:Our study, for the first time, identified a CREC strain that co-produces blasubNDM-1/sub and blasubIMP-4/sub in the Northeast China. Our finding emphasizes an urgent need for more intensive surveillance and precaution measures to prevent the CERC spread.
机译：背景：肠杆菌Cloacae复合物（ECC）是最常见的扩展β-内酰胺酶和碳碱酶促酶产病之一，威胁数百万老人和脆弱的病人。本研究的目的是在中国黑龙江省中出现的耐鲤鱼复合物（CREC）的分子特征。方法：从传染病患者中分离出六种CREC菌株。通过测序16S rRNA基因的聚合酶链反应（PCR）产物来确认ECC分离物的标识。通过测序Carbapenemase，AMPC和氟喹啉酮抗性基因的PCR产物进行CREC分离株的表征，并进行多点序列键入（MLST），脉冲场凝胶电泳（PFGE）和全基因组测序。结果：所有6个分离物都覆盖多个抗性基因。其中，5种载体 - β-内酰胺酶，一个是BLA KPC-2 - 阳性。左氧氟沙星和环丙沙星抗性菌株在喹啉抗性测定区域中具有Gyra83，Gyra87和Parc80的替代品。 MLST分析显示，6个分离株属于五个序列类型（ST520，ST528，ST1119，ST1120和ST93），而隔离物的PFGE图案落入四个簇。发现菌株ST1120携带两种分离的质粒，其编码BLA Ndm-1 和BLA IMP-4 。结论：我们的研究首次鉴定了CREC应变共同生产BLA NDM-1 和BLA IMP-4 在中国东北部。我们的发现强调了迫切需要更加密集的监控和预防措施，以防止CERC传播。

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