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首页> 外文期刊>BMC Plant Biology >Salt-inducible expression of OsJAZ8 improves resilience against salt-stress
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Salt-inducible expression of OsJAZ8 improves resilience against salt-stress

机译：奥斯雅兹的盐诱导的表达改善了抗盐胁迫的韧性

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Productivity of important crop rice is greatly affected by salinity. The plant hormone jasmonate plays a vital role in salt stress adaptation, but also evokes detrimental side effects if not timely shut down again. As novel strategy to avoid such side effects, OsJAZ8, a negative regulator of jasmonate signalling, is expressed under control of the salt-inducible promoter of the transcription factor ZOS3–11, to obtain a transient jasmonate signature in response to salt stress. To modulate the time course of jasmonate signalling, either a full-length or a dominant negative C-terminally truncated version of OsJAZ8 driven by the ZOS3–11 promoter were expressed in a stable manner either in tobacco BY-2 cells, or in japonica rice. The transgenic tobacco cells showed reduced mortality and efficient cycling under salt stress adaptation. This was accompanied by reduced sensitivity to Methyl jasmonate and increased responsiveness to auxin. In the case of transgenic rice, the steady-state levels of OsJAZ8 transcripts were more efficiently induced under salt stress compared to the wild type, this induction was more pronounced in the dominant-negative OsJAZ8 variant. The result concluded that, more efficient activation of OsJAZ8 was accompanied by improved salt tolerance of the transgenic seedlings and demonstrates the impact of temporal signatures of jasmonate signalling for stress tolerance.
机译：重要作业稻米的生产力受盐度大大影响。植物激素Jasonate在盐胁迫适应中起着至关重要的作用，但如果不及时关闭，也会唤起有害的副作用。作为避免这种副作用的新策略，奥斯妥8，茉莉酸盐信号传导的负调节剂，在转录因子ZOS3-11的盐诱导促进剂的控制下表达，以响应于盐胁迫获得瞬时茉满面签名。为了调节茉莉酸甲酸信号传导的时间过程，在烟草含量-2细胞中或在粳稻中以稳定的方式表达ZOS3-11启动子驱动的全长或主要负C末端截短版奥氏菌α8 。转基因烟草细胞显示出降低盐胁迫适应下的死亡率和有效的循环。这伴随着对茉莉甲酸甲酯的敏感性降低并增加对蟾蜍素的反应性。在转基因大米的情况下，与野生型相比，盐胁迫下稳定态水平较高，在盐胁迫下更有效地诱导，在主导阴性OSJAZ8变体中，这种诱导更加明显。结果总结出来，奥司AZ8的更有效活化伴随着转基因幼苗的耐盐性，并证明了茉莉酸字信号传导对应力耐受性的时间差异的影响。

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