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首页> 外文期刊>Cancer Management and Research >LncRNA miR143HG Up-Regulates p53 In Endometrial Carcinoma By Sponging miR-125a
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LncRNA miR143HG Up-Regulates p53 In Endometrial Carcinoma By Sponging miR-125a

机译：LNCRNA miR143Hg通过冲压miR-125a来调节子宫内膜癌中的p53

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Introduction: MiR143HG is a recently identified tumor suppressor in bladder cancer. We performed bioinformatics prediction and found that miR143HG can form base pairs with miR-125a. This study was therefore carried out to explore the interaction between miR143HG and miR-125a in endometrial carcinoma (EC). Methods: Gene expression was analyzed by qPCR and Western blot. Interactions among genes were analyzed by over-expression experiments. Cell apoptosis after transfections was analyzed by cell apoptosis assay. Results: We found that the down-regulation of miR143HG in EC predicted poor survival. Bioinformatics analysis showed that miR-125a could bind miR143HG. In EC tissues, miR143HG was positively correlated with p53, not miR-125a. In EC cells, miR143HG and miR-125a over-expression failed to affect the expression of each other. However, miR143HG over-expression led to the up-regulated p53. MiR-125a over-expression played the opposite role and attenuated the effects of miR143HG over-expression. Cell apoptosis analysis showed that miR143HG and p53 over-expression led to an increased cell apoptotic rate. MiR-125a over-expression played the opposite role and attenuated the effects of miR143HG over-expression. Conclusion: MiR143HG may up-regulate p53 in EC by sponging miR-125a to promote cancer cell apoptosis.
机译：简介：MIR143HG是膀胱癌中最近鉴定的肿瘤抑制剂。我们进行了生物信息学预测，发现MiR143Hg可以形成MiR-125A的碱基对。因此，进行该研究以探讨内膜癌（EC）中miR143Hg和miR-125a之间的相互作用。方法：通过QPCR和Western印迹分析基因表达。通过过表达实验分析基因之间的相互作用。通过细胞凋亡测定分析转染后细胞凋亡。结果：我们发现欧共体中MiR143Hg的下调预测存活率差。生物信息学分析表明miR-125a可以结合mir143hg。在EC组织中，MIR143HG与P53，而不是miR-125a呈正相关。在EC细胞中，miR143Hg和miR-125a过表达未能影响彼此的表达。但是，MIR143HG过表达导致上调的P53。 miR-125a过表达起到了相反的作用，并减弱了miR143hg过表达的影响。细胞凋亡分析显示MiR143Hg和P53过表达导致细胞凋亡率增加。 miR-125a过表达起到了相反的作用，并减弱了miR143hg过表达的影响。结论：MIR143HG通过冲压miR-125a促进癌细胞凋亡的eC中的P53。

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