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首页> 外文期刊>Cancer Management and Research >Decreasing Microtubule Actin Cross-Linking Factor 1 Inhibits Melanoma Metastasis by Decreasing Epithelial to Mesenchymal Transition
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Decreasing Microtubule Actin Cross-Linking Factor 1 Inhibits Melanoma Metastasis by Decreasing Epithelial to Mesenchymal Transition

机译：减少微管肌动蛋白交联因子1通过减少上皮对间充质转换来抑制黑素瘤转移

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Background: The microtubule actin cross-linking factor 1 (MACF1) is involved in cellular migration, adhesion, and invasion processes. Its abnormal expression initiates tumor cell proliferation and metastasis in numerous cancer types. Methods: In this study, we utilized short hair-pin RNA interference of MACF1 to assess the inhibitory effects on the metastatic potential of B16F10 melanoma cells both in vitro and in vivo a mouse model. Results: The MACF1 expression was increased in B16F10 cells-induced tumor tissues; while the down-regulation of MACF1 impacted the B16F10 melanoma cell metastatic behavior by decreasing the ability of colony formation and invasion in vitro as well as inhibiting B16F10 cells-induced tumor growth and lung metastasis in vivo. The results of Western blot and immunohistochemistry indicated that the expression of E-cadherin and Smad-7 was significantly increased whereas the expression of N-cadherin and TGF-β 1 was significantly decreased in tumor tissue of mice challenged with the B16F10/MACF1-RNAi cells when compared with the B16F10 cells challenged mice. Conclusion: The data presented in this study demonstrated that down-regulated MACF1 expression decreased B16F10 melanoma metastasis in mice by inhibiting the epithelial to mesenchymal transition program. Thus, MACF1 may be a novel target for melanoma therapy.
机译：背景：微管肌动蛋白交联因子1（MACF1）涉及细胞迁移，粘附和侵袭过程。其异常表达引发了许多癌症类型中的肿瘤细胞增殖和转移。方法：在本研究中，我们利用MACF1的短发销RNA干扰，评估对体外和体内B16F10黑素瘤细胞的转移潜力的抑制作用。结果：B16F10细胞诱导的肿瘤组织中，MACF1表达增加;虽然MacF1的下调通过降低体外菌落形成和侵袭的能力影响B16F10黑色素瘤细胞转移行为，以及抑制B16F10细胞诱导的肿瘤生长和体内肺转移。蛋白质印迹和免疫组织化学的结果表明，E-钙粘蛋白和Smad-7的表达显着增加，而N-Cadherin和TGF-β1的表达在挑战B16F10 / MacF1-RNAi的小鼠肿瘤组织中显着降低与B16F10细胞攻击小鼠的小鼠相比的细胞。结论：本研究表明的数据证明，下调MACF1表达通过抑制下皮对间充质转换程序来降低小鼠的B16F10黑色素瘤转移。因此，MacF1可以是黑色素瘤治疗的新靶标。

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