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首页> 外文期刊>Canadian Respiratory Journal >Budesonide and Calcitriol Synergistically Inhibit Airway Remodeling in Asthmatic Mice
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Budesonide and Calcitriol Synergistically Inhibit Airway Remodeling in Asthmatic Mice

机译：预升尼蛋白和钙质协同抑制哮喘小鼠的气道重塑

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Background and Objective. While calcitriol can inhibit airway remodeling in asthmatic mice, the mechanism remains unclear. The purpose of this study was to explore the mechanism of action of calcitriol on airway remodeling in asthma and its interaction with budesonide. Methods. A mouse model of asthma was established by allergic sensitization and challenge with ovalbumin. The mice were treated with budesonide, calcitriol, or budesonide plus calcitriol. The expression of airway remodeling-related proteins, transforming growth factor β (TGFβ) signaling pathway-related proteins, the glucocorticoid receptor, and vitamin D receptor (VDR) was determined by immunohistochemical staining and Western blot analysis. Quantitative real-time PCR was used to determine the expression of microRNA-21 (miR-21) in the lung tissue of mice. Results. Monotherapy with budesonide or calcitriol inhibited the high expression of collagen type I protein and upregulated the low expression of Smad7 in asthmatic mice. There was a synergistic interaction between budesonide and calcitriol in combined treatment. The expression of miR-21 in the combined treatment group was significantly lower than that in the calcitriol treatment group. VDR expression in the combined treatment group was significantly higher than that of the calcitriol treatment group. Conclusion. Budesonide and calcitriol have a synergistic effect on airway remodeling in asthmatic mice.
机译：背景和目标。虽然CalcItal可以在哮喘小鼠中抑制气道重塑，但该机制仍不清楚。本研究的目的是探讨亚硝醇对哮喘呼吸道重塑的作用机制及其与预烯烷的相互作用。方法。通过过敏性敏化和卵泡攻击建立了一种哮喘模型。将小鼠用果冻，氧化二醇或果仁烷烃加上钙质。通过免疫组织化学染色和蛋白质印迹分析测定气道重塑相关蛋白质，转化生长因子β（TGFβ）与维生素D受体（VDR）的表达。定量实时PCR用于确定小鼠肺组织中MicroRNA-21（miR-21）的表达。结果。单药治疗含有蛋白质或钙质的蛋白质蛋白酶I蛋白的高表达，并在哮喘小鼠中上调了Smad7的低表达。在组合治疗中，预烯胺和钙质之间存在协同相互作用。联合治疗组中miR-21的表达明显低于钙质处理基团中的miR-21。组合治疗组中的VDR表达明显高于钙质处理组。结论。预烯胺和钙质对哮喘小鼠的气道重塑具有协同作用。

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