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A ratiometric fluorescent probe for real-time monitoring of intracellular glutathione fluctuations in response to cisplatin

机译：一种比例荧光探针，用于实时监测响应顺铂的细胞内谷胱甘肽波动

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Real-time imaging of fluctuations in intracellular glutathione (GSH) concentrations is critical to understanding the mechanism of GSH-related cisplatin-resistance. Here, we describe a ratiometric fluorescence probe based on a reversible Michael addition reaction of GSH with the vinyl-functionalized boron-dipyrromethene (4,4-difluoro-4-bora-3 a ,4 a -diaza- s -indacene or BODIPY) 1 . The probe was applied for real-time monitoring of the fluctuations in GSH levels in cells under cisplatin treatment. Notably, in cellular cisplatin-sensitive A549 cells, GSH concentrations rose until cell death, while in cisplatin-resistant cell lines, GSH levels first rose to the maximum then fell back to the initial concentration without significant apoptosis. These results indicate that different trends in GSH fluctuation can help distinguish cisplatin-resistant from cisplatin-sensitive cells. As such, this study has shown that probe 1 may potentially be used for real-time monitoring of intracellular GSH levels in response to therapeutics.
机译：细胞内谷胱甘肽（GSH）浓度波动的实时成像对于了解GSH相关的顺铂抗性机理至关重要。这里，我们描述了基于GSH的可逆迈克尔加成反应的比例荧光探针与乙烯基官能化硼 - 二溴乙烯（4,4-二氟-4-硼-3a，4A -diaza-indacene或Bodipy） 1。探针应用于顺铂治疗下细胞中GSH水平波动的实时监测。值得注意的是，在细胞顺铂敏感性A549细胞中，GSH浓度升高至细胞死亡，而在顺铂抗性细胞系中，GSH水平首先升至最大升至初始浓度无明显凋亡。这些结果表明，GSH波动的不同趋势可以帮助区分顺铂敏感细胞的顺铂。因此，该研究表明，探针1可能用于响应于治疗剂的细胞内GSH水平的实时监测。

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