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Simple Simultaneous Assay of Methotrexate and Non-Steroidal Anti-Inflammatory Drugs by HPLC

机译：HPLC简单同时测定甲氨蝶呤和非甾体类抗炎药物

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The combination of methotrexate (MTX) and non-steroidal anti-inflammatory drugs (NSAIDs), which is frequently used for rheumatoid arthritis (RA) treatment, courses of adverse events. To prevent these, simultaneous monitoring of these compounds is available. Therefore, we developed a new method for MTX and NSAIDs such as loxoprofen (LP), meloxicam (MX), lornoxicam (LX), diclofenac (DF) and celecoxib (CX) determination using HPLC with simple pretreatment of human serum sample. The separation of MTX and 5 NSAIDs was performed on C6-Phenyl column with gradient elution using 10 mmol/L ammonium acetate aqueous solution and methanol, and achieved within 25 min of analytical runtime. Calibration curves using standards showed good linearity (r ~(2 )>0.9998) in the range of 0.02-500 pmol/10 μL injection for MTX, 10-500 pmol for LP, 0.2-500 pmol for MX and DF, 0.05-500 pmol for LX and 2-500 pmol for CX. The detection limits of the proposed method were at least less than 96.0 fmol, and repeatability was less than 4.62 RSD%. In addition, acceptable precision of less than 10.19 RSD% and recovery of more than 54.4% of the method were obtained because the peaks of MTX and NSAIDs could be well-separated from those of interferings in serum. Therefore, the proposed method might be useful to avoid occurring adverse events, and confirm the curative effect of MTX and NSAIDs.
机译：甲氨蝶呤（MTX）和非甾体抗炎药（NSAID）的组合，其经常用于类风湿性关节炎（RA）治疗，不良事件的疗程。为了防止这些化合物的同时监测。因此，我们为使用HPLC具有简单预处理人血清样品的MTX和NSAID，如洛杉矶洛芬（LP），Meloxicam（MX），洛杉矶昔康（LX），双氯芬（DF）和Celecoxib（CX）测定的新方法。在C6-苯基柱上进行MTX和5 nSAID的分离，使用10mmol / L乙酸铵水溶液和甲醇进行梯度洗脱，并在分析运行时间的25分钟内实现。使用标准的校准曲线显示出良好的线性度（ R〜（ 2）> 0.9998），其MTX，10-500pmol用于LP的10-500pmol，MX为0.2-500pmol的范围内的0.02-500pmol /10μl注射液和DF，用于CX的LX和2-500pmol的0.05-500pmol。所提出的方法的检测限率至少小于96.0氟醇，可重复性小于4.62克劳德％。此外，获得的可接受精度低于10.19克朗％并恢复超过54.4％的方法，因为MTX和NSAIDS的峰可以与血清中干扰的峰分离。因此，所提出的方法可能有助于避免发生不良事件，并确认MTX和NSAID的疗效。

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