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首页> 外文期刊>CHROMATOGRAPHY >Simultaneous Determination of 7 Short-Chain Fatty Acids in Human Saliva by High-Sensitivity Gas Chromatography-Mass Spectrometry
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Simultaneous Determination of 7 Short-Chain Fatty Acids in Human Saliva by High-Sensitivity Gas Chromatography-Mass Spectrometry

机译：高灵敏度气相色谱 - 质谱法同时测定人类唾液中的7个短链脂肪酸

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Upon absorption in the intestine of the host animal, the main function of short-chain fatty acids (SCFAs), mainly acetate, propionate and n-butyrate, is as metabolic energy. SCFAs, n-butyrate in particular, can also be found in the mouth. An excess of oral SCFAs may cause not only periodontal diseases but also systemic abnormalities in humans. Previously, we reported a method for simultaneous detection by gas chromatography-mass spectrometry (GC-MS) of acetate, propionate and n-butyrate in serum, urine and saliva. In the present study we used a modified version of this method to detect not only acetate, propionate and n-butyrate, but also iso-butyrate, n-valerate, iso-valerate and caproate, because the latter are suggested to be associated with periodontal diseases. Detection ranges of SCFAs were as follows; 6.25-400 μmol/L (acetate), 0.781-100 μmol/L (propionate and n-butyrate), 0.391-50 μmol/L (iso-butyrate), 0.781-50 μmol/L (n-valerate and iso-valerate) and 1.56-50 μmol/L (caproate). Furthermore, we validated the modified detection method with triple freeze-thaw-cycle recovery tests and intra- and inter-day repeatability. Freezing and thawing did not influence the concentrations of SCFAs in saliva. Upon analysis of five clinical saliva samples, it was observed that, except for n-valerate, which was detected only in two samples, all SCFAs were detected in saliva samples. To conclude, we were able to use a modified method to analyze successfully by GC-MS the salivary concentrations of SCFAs. In addition, we simultaneously detected the salivary concentrations of iso-butyrate, iso-valerate, nvalerate and caproate. This improved method was proved to be reliable to measure the concentrations of SCFAs in saliva.
机译：在宿主动物的肠道中吸收时，短链脂肪酸（SCFA）的主要功能，主要是醋酸盐，丙酸盐和正丁酸丁酸酯是代谢能。 SCFA，特别是N-丁酸盐，也可以在口中找到。过量的口服SCFA可能导致人类的牙周病，而且可能导致人类的全身异常。以前，我们报道了一种通过在血清，尿液和唾液中通过气相色谱 - 质谱（GC-MS）同时检测乙酸盐，丙酸盐和正丁酸酯的方法。在本研究中，我们使用这种方法的改性版本，不仅检测醋酸盐，丙酸酯和正丁酸酯，还可以检测异丁酸酯，N-戊酸酯，异戊酯，因为后者被建议与牙周相关联疾病。 SCFA的检测范围如下; 6.25-400μmol/ l（醋酸盐），0.781-100μmol/ l（丙酸丁酸酯），0.391-50μmol/ L（异丁酸酯），0.781-50μmol/ L（N-戊符和异戊酸）和1.56-50μmol/ L（辣椒）。此外，我们验证了具有三重冻融循环恢复试验的改进的检测方法以及日内和日内的可重复性。冷冻和解冻没有影响唾液中SCFA的浓度。在分析五个临床唾液样品后，观察到，除N-戊酯外仅在两个样品中检测到，在唾液样品中检测到所有SCFA。为了得出结论，通过GC-MS的SCFA的唾液浓度，我们能够使用修改方法来分析。此外，我们同时检测了异丁酸酯，异戊酯，甲苯二甲酸盐的唾液浓度。证明这种改进的方法可靠地测量唾液中SCFA的浓度。

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